1. MCE Kits
  2. Protein Biology
  3. Protein Electrophoresis & WB
  4. Chemiluminescent Detection
  5. High Sensitivity ECL Kit

High Sensitivity ECL Kit 

Cat. No.: HY-K2005
Manual SDS

High Sensitivity ECL Kit enables low picogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems.

High Sensitivity ECL Kit
Size Price Stock Quantity
Free Sample (10 mL)   Apply Now  
100 mL USD 68 In-stock
500 mL USD 243 In-stock

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE High Sensitivity ECL Kit enables low picogram detection of antigen by oxidizing luminol in the presence of HRP and peroxide. This reaction produces a prolonged chemiluminescence which can be visualized on X-ray film or digital imaging systems.

The Kit produces a strong, long-lived signal (6-8 hours), which, combined with very low background levels, allows for long exposure times and enables the detection of low-abundance proteins. In the Western Blot experiment, the storage solution of primary antibody (1 mg/mL) can be diluted at a ratio of 1:1,000-1:50,000. (the final concentration is about 0.2-1 μg/mL). The secondary antibody (1 mg/mL) storage solution can be diluted at a ratio of 1:20,000-1:100,000 (the final concentration is about 10-50 ng/mL).

Publications
Storage

4°C, 1 year.

Protect from light

Components
Components HY-K2005-100 mL HY-K2005-500 mL
Solution A 50 mL 50 mL × 5
Solution B 50 mL 50 mL × 5
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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High Sensitivity ECL Kit
Cat. No.:
HY-K2005
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