CEPT Cocktail
Based on 9 publication(s) in Google Scholar
MCE CEPT Cocktail is composed of CET small molecules (Chroman 1, Emricasan and Trans-ISRIB) and Polyamine Solution, and can effectively enhance cell survival and promote clonal growth of genetically stable hPSCs.
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Storage :
-20°C, 2 years.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE CEPT Cocktail is composed of CET small molecules (Chroman 1, Emricasan and Trans-ISRIB) and Polyamine Solution. This product enhances cell survival and promotes clonal growth of genetically stable hPSCs. In addition, this product provides strong improvements for several key applications in stem cell research, including routine cell passaging, cryopreservation of pluripotent and differentiated cells, embryoid body (EB) and organoid formation, etc.
Protocol
1. Preparation of stock solutions
| Component | Stock preparation | Stock concentration | Working concentration |
|---|---|---|---|
| Chroman 1 | Dissolve 5 mg in 22.91 mL DMSO and vortex until completely dissolved | 0.5 mM (10,000×) | 50 nM |
| Emricasan | Dissolve 10 mg in 0.3512 mL DMSO and vortex until completely dissolved | 50 mM (10,000×) | 5 μM |
| Trans-ISRIB | Dissolve 10 mg in 3.165 mL DMSO; gently warm at 45-60°C and vortex until completely dissolved | 7 mM (10,000×) | 0.7 μM |
| Polyamine Solution | Supplied with the kit | 1,000× | 1× |
2. Preparation of CEPT-containing culture medium
Add Chroman 1, Emricasan, and Trans-ISRIB stocks to complete hPSC culture medium at 1 : 10,000 immediately before use. For example, add 1 μL of each 10,000× stock to 10 mL complete medium.
3. Add Polyamine Solution at 1 : 1,000. For example, add 10 μL Polyamine Solution (1,000×) to 10 mL complete medium.
4. Mix thoroughly and sterile-filter through a 0.22 μm filter. The final CEPT medium contains 50 nM Chroman 1, 5 μM Emricasan, 0.7 μM Trans-ISRIB, and 1× Polyamine.
Note: CEPT Cocktail can be used for routine hPSC passaging, single-cell cloning, cryopreservation/recovery, EB formation, organoid formation, and related applications. Use all four components together for optimal cytoprotection.
Publications
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Journal Impact Factor
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Most Recent
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Cell Stem CellSignaling reprogramming via Stat3 activation unravels high-fidelity human post-implantation embryo modeling. [Abstract]2025 Oct 2;32(10):1528-1544.e10. PMID: 40961945
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Cell Stem CellModeling early gastrulation in human blastoids with DNA methylation patterns of natural blastocysts. [Abstract]2025 Mar 6;32(3):409-425.e8. PMID: 39814012
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iScienceUnperturbed dye-based imaging of spontaneous synchronized calcium activity in iPSC-derived neuronal cultures. [Abstract]2026 Apr 9;29(5):115689. PMID: 42080132
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bioRxivAttenuated estrogen signaling disrupts placentation and drives trophoblast defects in Down syndrome. [Abstract]2026 Jun 8:2026.06.03.729739. PMID: 42327050
Storage
-20°C, 2 years.
Components
| Components | HY-K1043 |
|---|---|
| Chroman 1 | 5 mg |
| Emricasan | 10 mg |
| Trans-ISRIB | 10 mg |
| Polyamine Solution (1,000×) | 5 mL |
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