1. MCE Kits
  2. Molecular Biology
  3. PCR & qPCR
  4. Polymerase Chain Reaction (PCR)
  5. 2× High-Fidelity PCR Master Mix

2× High-Fidelity PCR Master Mix 

Cat. No.: HY-K0533
Manual SDS

2× High-Fidelity PCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for PCR except sample DNA, primers and water.

2× High-Fidelity PCR Master Mix
Size Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 58 In-stock
5 mL USD 207 In-stock

* Please select Quantity before adding items.

  • Description

  • Storage

  • Components

  • Documentation

& Advantages

MCE 2× High-Fidelity PCR Master Mix is a new high-fidelity PCR amplification premix. The product contains a new type of modified high-fidelity DNA Polymerase with higher 5’→3’polymerase activity and higher 3’→5’exonuclease activity. The additional hot-start factor can be activated to improve specificity and stability of PCR amplification by minimizing the non-specific amplification and enzyme activity loss. The product also contains a unique Extension-Enhancement factor, making the extending speed and the amplification efficient significantly improved. It can effectively amplify the fragments up to 10 kb, and the amplification speed can reach 15 sec/kb.

MCE 2× High-Fidelity PCR Master Mix is a ready-to-use premix. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, and provides consistent reaction-to-reaction performance. This product has no DNA loading buffer, the DNA loading buffer should be added to the PCR product when run on the agarose gel. The PCR product is blunt-ended.


Store at -20°C for 2 years. 4°C for short-term storage (up to 3 months). Avoid repetitive freeze-thaw cycles while using.

Components HY-K0533- 1 mL HY-K0533- 5 mL
2× High-Fidelity PCR Master Mix 1 mL 1 mL × 5
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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