2× High-Fidelity PCR Master Mix

MCE 2× High-Fidelity PCR Master Mix is a new high-fidelity PCR amplification premix. The product contains a new type of modified high-fidelity DNA Polymerase with higher 5’→3’polymerase activity and higher 3’→5’exonuclease activity. The additional hot-start factor can be activated to improve specificity and stability of PCR amplification by minimizing the non-specific amplification and enzyme activity loss. The product also contains a unique Extension-Enhancement factor, making the extending speed and the amplification efficient significantly improved. It can effectively amplify the fragments up to 10 kb, and the amplification speed can reach 15 sec/kb.

MCE 2× High-Fidelity PCR Master Mix is a ready-to-use premix. With the primers and template added, the optimized system will provide sensitive and reliable DNA synthesis. It saves preparation time, reduces the risk of contamination from multiple pipetting steps, and provides consistent reaction-to-reaction performance. This product has no DNA loading buffer, the DNA loading buffer should be added to the PCR product when run on the agarose gel. The PCR product is blunt-ended.