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  6. Bi-27 Serum-free Supplement (50×), Insulin-Free

Bi-27 Serum-free Supplement (50×), Insulin-Free 

Cat. No.: HY-K3014C
Manual COA Technical Support

MCE Bi-27 Serum-free Supplement (50×), Insulin-Free is a customized formulation developed by removing insulin from the standard Bi-27 formulation. As a neuronal cell culture supplement, it is specifically designed to support the growth and maintenance of hippocampal neurons and other central nervous system (CNS) neurons in applications where the presence of insulin may interfere with experimental outcomes.

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50 mL Ask For Quote & Lead Time

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    MedChemExpress Validation
    Immunofluorescence (IF) analysis of SD rat cortical neurons: After 7 d of in vitro culture (10× magnification), three culture conditions were compared: no Bi-27 supplement (W/O), Bi-27 Serum-free Supplement (50×), Insulin-Free (HY-K3014C), and Gibco B-27 Supplement, minus insulin (Competitor). The results demonstrated that supplementation with Bi-27 significantly enhanced neuronal attachment, increased cell density, and promoted more extensive and well-developed neurite outgrowth. In contrast, the W/O group showed poor overall cell condition, characterized by reduced adhesion, decreased cell density, and limited neurite development. Further comparison indicated that HY-K3014C and the Gibco B-27 Supplement, minus insulin (Competitor), exhibited comparable performance in supporting the growth and differentiation of SD rat cortical neurons, with no statistically significant differences observed between the two groups. Overall, HY-K3014C demonstrated equivalent performance to the imported competitor in maintaining neuronal morphology, promoting cell growth, and supporting neurite development.

    MedChemExpress Validation
    Analysis of CTG assay and microscopic observation in colon cancer cells: After 6 days of in vitro culture, three culture groups were compared: no Bi-27 supplement (W/O), Bi-27 Serum-free Supplement (50×), Insulin-Free (HY-K3014C), and Gibco B-27 Supplement, minus insulin (Competitor). Cell growth status was comprehensively evaluated by microscopic observation (4×, 10×, 20× magnification) and CellTiter-Glo (CTG) luminescence detection. The results showed that compared with the Bi-27-free control group, supplementation with insulin-free B-27 improved the overall cellular growth, featuring more intact cell structure, clearer morphology of organoids/cell clusters and a more obvious increase in volume. Quantitative CTG results further revealed no statistically significant differences in cell viability among all groups (ns). Specifically, HY-K3014C and Gibco B-27 Supplement, minus insulin (Competitor) exerted equivalent effects on cell viability, with neither showing a significant difference relative to the control group. In conclusion, in this colon cancer cell culture system, HY-K3014C Bi-27 performs comparably to the competitor in supporting cell growth and preserving cell viability, with no remarkable functional differences detected.

    • Description

    • Storage

    • Protocol

    • Attention

    • Components

    • Documentation

    Description
    & Advantages

    MCE Bi-27 Serum-free Supplement (50×), Insulin-Free is a customized formulation developed by removing insulin from the standard Bi-27 formulation. As a neuronal cell culture supplement, it is specifically designed to support the growth and maintenance of hippocampal neurons and other central nervous system (CNS) neurons in applications where the presence of insulin may interfere with experimental outcomes.

    This product is an optimized serum-free supplement formulation enriched with multiple antioxidants to help reduce oxidative stress-induced cellular damage, thereby improving neuronal viability and culture stability.

    The recommended working concentration of Bi-27 Supplement is 2% (v/v), corresponding to a 1:50 dilution before use in the culture medium.
    Storage

    -20°C, 2 years

    Keep away from light

    Shipping with dry ice
    Protocol

    Prepare complete Neuronal basal Medium

    1. Thaw MCE Bi-27 Serum-free Supplement (50×), Insulin-Free overnight at 4°C.

    2. Aseptically add 2% Bi-27 and 0.5mM L-Glutamine to the Neuronal Basal Medium before use.

    3. Primary Hippocampal Neuron Culture: Add 25 µM of L-glutamate to the complete Neuronal Basal Medium. The medium can be replaced after 4 days of culture, and glutamate should not be added to the medium after this change.

    Note: Once prepared, the complete Neuronal Basal Medium can be stored at 4°C for two weeks, protected from light.
    Attention

    1. This product appears as a light yellow liquid. The color may not be consistent following freezing, but it can fully dissolve in a 37°C water bath.

    2. It is recommended to appropriately aliquot the solution to avoid repeat freeze-thaw cycles.

    3. Pay attention to aseptic operation to avoid contamination.

    4. This product is for R&D use only, not for drug, household, or other uses.

    5. For your safety and health, please wear a lab coat and disposable gloves to operate.
    Components
    Components HY-K3014C-10 mL HY-K3014C-50 mL
    Bi-27 Serum-free Supplement (50×), Insulin-Free 10 mL 10 mL × 5
    Documentation

    Manual (1328 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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