Bi-27 Serum-free Supplement (50×), Insulin-Free
MCE Bi-27 Serum-free Supplement (50×), Insulin-Free is a customized formulation developed by removing insulin from the standard Bi-27 formulation. As a neuronal cell culture supplement, it is specifically designed to support the growth and maintenance of hippocampal neurons and other central nervous system (CNS) neurons in applications where the presence of insulin may interfere with experimental outcomes.
-
Storage :
-20°C, 2 years. Keep away from light. Shipping with dry ice.
- Manual (1328KB)
- COA
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE Bi-27 Serum-free Supplement (50×), Insulin-Free is a customized formulation developed by removing insulin from the standard Bi-27 formulation. As a neuronal cell culture supplement, it is specifically designed to support the growth and maintenance of hippocampal neurons and other central nervous system (CNS) neurons in applications where the presence of insulin may interfere with experimental outcomes.
This product is an optimized serum-free supplement formulation enriched with multiple antioxidants to help reduce oxidative stress-induced cellular damage, thereby improving neuronal viability and culture stability.
The recommended working concentration of Bi-27 Supplement is 2% (v/v), corresponding to a 1:50 dilution before use in the culture medium.
Protocols
Prepare complete Neuronal basal Medium
1. Thaw MCE Bi-27 Serum-free Supplement (50×), Insulin-Free overnight at 4°C.
2. Aseptically add 2% Bi-27 and 0.5mM L-Glutamine to the Neuronal Basal Medium before use.
3. Primary Hippocampal Neuron Culture: Add 25 µM of L-glutamate to the complete Neuronal Basal Medium. The medium can be replaced after 4 days of culture, and glutamate should not be added to the medium after this change.
Note: Once prepared, the complete Neuronal Basal Medium can be stored at 4°C for two weeks, protected from light.
Storage
-20°C, 2 years.
Keep away from light.
Shipping with dry ice.
Attention
1. This product appears as a light yellow liquid. The color may not be consistent following freezing, but it can fully dissolve in a 37°C water bath.
2. It is recommended to appropriately aliquot the solution to avoid repeat freeze-thaw cycles.
3. Pay attention to aseptic operation to avoid contamination.
4. This product is for R&D use only, not for drug, household, or other uses.
5. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K3014C-10 mL | HY-K3014C-50 mL |
|---|---|---|
| Bi-27 Serum-free Supplement (50×), Insulin-Free | 10 mL | 10 mL × 5 |
Please enter the correct Lot Number
