Advanced DMEM Reduced-Serum Medium, no L-Glutamine, no HEPES
MCE Advanced DMEM Reduced-Serum Medium (without L-Glutamine or HEPES) is a mammalian cell culture medium optimized from the classic high-glucose DMEM formulation for reduced-serum culture.
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Storage :
2–8°C, 1 year. Protect from light.
- Manual (1660KB)
- COA
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Description & Advantages
MCE Advanced DMEM Reduced-Serum Medium (without L-Glutamine or HEPES) is a mammalian cell culture medium optimized from the classic high-glucose DMEM formulation for reduced-serum culture. Compared with conventional DMEM, it allows the amount of fetal bovine serum (FBS) added to be reduced by 50%–90% while maintaining cell growth rate and morphology. This can reduce variation in culture conditions caused by differences between serum lots, extend the use of each serum lot, and provide a more consistent and economical option for cell culture.
| (+) | (-) |
|---|---|
| D-Glucose (4.5 g/L) | L-Glutamine |
| Non-essential amino acids (NEAA) | HEPES |
| Phenol Red (15 mg/L) | |
| Sodium Pyruvate (110.0 mg/L) |
Protocols
MCE Advanced DMEM Reduced-Serum Medium (without L-Glutamine or HEPES) contains ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin, and trace elements, including sodium selenite, ammonium metavanadate, copper sulfate, and manganese chloride. These components help reduce the amount of fetal bovine serum required while maintaining normal cell growth rate and morphology. Before use, aseptically add 1%–5% fetal bovine serum (FBS) and supplement with either L-Glutamine (Cat. No.: HY-K1046) or a stabilized L-Alanyl-L-Glutamine supplement (Cat. No.: HY-K1046P) to a final concentration of 4 mM. Mix thoroughly before use.
Application
MCE Advanced DMEM Reduced-Serum Medium (without L-Glutamine or HEPES) is suitable for culturing a wide range of commonly used cell lines, including MDBK, MDCK, Vero, COS-7, A549, 3T3-L1, HuH-7, MDA-MB-231, SW620, NRK-52E, A-673, C2C12, RH-35, Ishikawa, and CHL. It can be used for routine cell maintenance, passaging, and large-scale culture.
Storage
2–8°C, 1 year.
Protect from light.
Attention
1. This product has been sterilized by filtration. Use aseptic technique to avoid contamination.
2. Not all cells are suitable for reduced-serum culture. Before switching to this medium, perform a small-scale preliminary test to assess cell morphology, viability, and proliferation. Optimize the fetal bovine serum concentration for each cell line, reducing serum use as much as possible while maintaining normal cell growth.
3. When using this product for cell culture, place the cells in a temperature-controlled incubator with 5%–10% CO₂ to maintain an appropriate growth environment.
4. This product is intended for scientific research by trained personnel only. It must not be used for clinical diagnosis or treatment, or in food or pharmaceutical products.
5. For your safety and health, wear a lab coat and disposable gloves when handling this product.
Components
| Components | HY-K3043-500 mL | HY-K3043-3 L |
|---|---|---|
| Advanced DMEM Reduced-Serum Medium, no L-Glutamine, no HEPES | 500 mL | 500 mL×6 |
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