2. Enzyme
  3. Tool Enzyme

Tool Enzyme

There are many types of tool enzymes with different functions, including common polymerases, reverse transcriptases, modifying enzymes, etc.

Tool enzymes are mainly used for:

• Molecular biology research

• mRNA synthesis

Tool Enzyme (42):

Cat. No. Product Name CAS No.  
  • HY-E70097
    Sialidase (α2-3-6-8-9)  
    Sialidase (α2-3-6-8-9) is a broadly specific sialidase that cuts linear and branched non-reducing terminal sialic acid residues from glycoproteins, glycopeptides, and oligosaccharides. Sialidase (α2-3-6-8-9) can be used for in vitro and in vivo polysaccharide analysis and characterization as well as complete glycoprotein remodeling.
  • HY-E70086
    Taq DNA polymerase 2304873-37-6  
    Taq DNA polymerase is a thermostable DNA polymerase that can be used in PCR.
  • HY-E70090
    T7 RNA polymerase 9014-24-8  
    T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter.
  • HY-E70094
    T4 UvsX Recombinase  
    T4 UvsX Recombinase helps initiate DNA replication on a double-stranded DNA template by catalyzing synapsis between the template and a homologous DNA single strand that serves as primer. T4 UvsX Recombinase greatly amplifies the snap-back (hairpin-primed) DNA synthesis that is catalyzed by the T4 DNA polymerase holoenzyme on linear, single-stranded DNA templates.
  • HY-E70373
    λ Protein phosphatase 401941-75-1  
    λ Protein phosphatase is a serine/threonine phosphatase encoded by bacteriophage Lambda. λ Protein phosphatase is activated with requirement for divalent cations, such as Mn2+. λ Protein phosphatase is able to dephosphorylate casein, adenovirus E1A protein, and the α subunit of phosphorylase kinase
  • HY-E71227
    α-Factor-transporting ATPase  
    α-Factor-transporting ATPase (EC 7.4.2.7) is a plasma membrane ATP-binding cassette (ABC) transporter that actively exports the farnesylated lipopeptide mating pheromone a-factor from the cytosol of MATa haploid cells.
  • HY-E71303
    β-Glucan-transporting ATPase  
    β-Glucan-transporting ATPase (EC 7.5.2.3) can be used to investigate the application of β-glucan-transporting ATPase in carbohydrate transport research.
  • HY-E70904
    Terminal Transferase, Calf  
    Terminal Transferase, Calf (EC 2.7.7.31) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for Terminal Transferase. Terminal Transferase does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
  • HY-W011134
    Palmitic acid N-hydroxysuccinimide 14464-31-4  
    Palmitic acid N-hydroxysuccinimide is used to conjugate proteins to prepare targeted delivery vectors. Palmitic acid N-hydroxysuccinimide can be used as lipophilic electrophile. Palmitic acid N-hydroxysuccinimide can be used for the covalent connection between palmitic acid and ovalbumin. Palmitic acid N-hydroxysuccinimide can be used to synthesize cetacyl derivatives of amino acids, aminoacyl-trNA, coenzyme A, mercaptoacetic acid and other amino and thiogenic compounds.
  • HY-131161
    M-MLV Reverse transcriptase 9068-38-6  
    M-MLV Reverse transcriptase (M-MLV RT) is a kind of Reverse transcriptase, from the moroni mouse leukemia virus (MoMLV).
  • HY-E70376
    Pfu DNA Polymerase 2304873-45-6  
    Pfu DNA Polymerase is an important enzyme in PCR-related experiments, which is initially found in Pyrococcus furiosus. Pfu DNA Polymerase exhibits thermostability and high fidelity.
  • HY-W424779
    5-Carboxy-2′-deoxyuridine 14599-46-3  
    5-Carboxy-2′-deoxyuridine is a metabolite of Trifluridine. 5-Carboxy-2′-deoxyuridine is a methyl oxidation product of Thymidine that can be formed by menadione-mediated photosensitization of Thymidine.
  • HY-E70421
    Peptide ligase  
    Butelase 1 ligase can be used for the cyclization of food-derived angiotensin I converting enzyme (ACE) inhibitory peptides, and thus improves the stability and bioactivity of ACE inhibitory peptides.
  • HY-E70095
    T4 UvsY Protein  
    T4 UvsY Protein is an accessory protein for in vitro catalysis of strand exchange. T4 UvsY Protein enhances strand exchange by UvsX protein by interacting specifically with UvsX protein. UvsY protein enhances the rate of single-stranded-DNA-dependent ATP hydrolysis by UvsX protein.
  • HY-P3005
    T4 DNA ligase 9015-85-4  
    T4 DNA ligase is the product of gene 30 of phage T4. T4 DNA ligase catalyzes the repair of single-stranded nicks in duplex DNA and joins duplex DNA restriction fragments having either blunt or cohesive ends. T4 DNA ligase catalyze the sealing of adjacent 5′-phosphate and 3′-­hydroxyl termini at single-stranded breaks in double-stranded DNA.T4 DNA ligase is an ATP-dependent ligase enzyme. T4 DNA ligase can be used in various biotechnological applications. T4 DNA ligase can join the ends of single-stranded DNA in the absence of any duplex DNA structure at the ligation site.
  • HY-P3260
    5'-Nucleotidase, Microorganism 9027-73-0  
    5′-Nucleotidase, Microorganism (CD73) is an intrinsic membrane glycoprotein present as an ectoenzyme. 5′-Nucleotidase catalyzes hydrolysis of 5-nucleotides to their corresponding nucleosides.
  • HY-E70404
    FastTaq DNA Polymerase(5'→3' exo-)  
    FastTaq DNA Polymerase (5'→3' exo-) is a modified DNA polymerase based on Taq DNA Polymerase. FastTaq DNA Polymerase (5'→3' exo-) lacks the 5'→3' exonuclease activity of wild-type Taq. It retains the 5'→3' DNA polymerase activity of wild-type Taq.
  • HY-E70081
    mRNA Cap 2'-O-methyltransferase  
    mRNA Cap 2'-O-methyltransferase uses S-adenosylmethionine (SAM) as a methyl donor to add a methyl group at the 2'-O position of the first nucleotide at the 5’ end of Cap-0 mRNA, resulting in Cap-1 structure. Cap-1 structure promotes translation efficiency, increasing subsequent protein expression.
  • HY-E70080
    Vaccinia virus capping enzyme  
    Vaccinia virus capping enzyme is a transcription initiation factor. Vaccinia virus capping enzyme is a heterodimer of D1 (844 aa) and D12 (287 aa) polypeptides that executes all three steps in m7GpppRNA synthesis. Vaccinia virus capping enzyme has been used widely as a reagent for capping and cap-labeling RNAs in vitro.
  • HY-E70016
    Terminal deoxyribonucleotidyltransferase 9027-67-2  
    Terminal deoxyribonucleotidyltransferase (TdT) catalyses the condensation of deoxyribonucleotide triphosphates onto the 3' hydroxyl ends of DNA strands and adds N-regions to gene segment junctions during V(D)J recombination. Terminal deoxyribonucleotidyltransferase is expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphoma cells.