Taq DNA polymerase
Based on 1 Customer Validation
Taq DNA polymerase is a thermostable DNA polymerase that can be used in PCR.
For research use only. We do not sell to patients.
- CAS No.: 2304873-37-6
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
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DNA Polymerase |
Product Information
This product is produced by modifying the protein molecule of wild-type Taq DNA polymerase and expressing it in E. coli to enhance the enzyme's anti-interference ability.
The storage buffer: 20 mM Tris, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% NP-40, 0.5% Tween-20, 50% Glycerol, pH8 .0.
Reaction buffer: 100 mM Tris, 500 mM KCl, 15 mM MgCl2, 0.8% NP-40, pH 8.8
PCR amplification directly from whole blood
1. PCR system
2.5 μL reaction buffer, up to 5 μL whole blood DNA template, 2.5 μL 2.5 mM dNTP, 1 μL upstream primer (10 μM), 1 μL downstream primer (10 μM), 0.5 μL Taq DNA polymerase, and Nuclease-free water to make up the volume to 25 μL.
2. PCR cycle
1) 95°C 3min cycle 1 time;
2) 94°C 30sec, 55°C 30sec, 72°C 45-90sec, cycle 35-40 times;
3) 72°C 7min cycle 1 time.
| Specification | Type | Components | Volume | Quantity |
| 500 U | A | Taq DNA polymerase | 500 U | 1 |
| 500 U | B | 10 × Taq Buffer | 1 mL | 2 |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
≥50 U/μL
One unit is defined as the amount of enzyme that catalyze the incorporation of 1 nmol AMP into the polynucleotide at 37°C in 60 min.
Chemical Information
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CAS No. 2304873-37-6
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Taq DNA polymerase]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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