SDS-PAGE Protein Loading Buffer (non-Reducing, 5×)
Based on 1 Customer Validation
MCE SDS-PAGE Protein Loading Buffer (non-Reducing, 5×) is appropriate for loading protein samples on SDS-PAGE. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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Storage :
-20℃, 2 years
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Description & Advantages
MCE SDS-PAGE Protein Loading Buffer (non-Reducing, 5×) is appropriate for loading protein samples on SDS-PAGE.
This product is a 5× concentrated loading buffer, containing the tracking dye bromophenol blue, which allows for monitoring the progress of electrophoresis. It contains denaturants such as SDS, without reducing agents such as DTT or 2-mercaptoethanol. After electrophoresis, the protein bands can be clearly visualized using staining methods such as Coomassie Brilliant Blue or silver staining. The migration speed of proteins is inversely proportional to its molecular weight, the smaller the molecular weight, the faster the migration speed.
Protocol
1. Dissolve the SDS-PAGE Protein Loading Buffer (non-Reducing, 5×) at room temperature or in a water bath not exceeding 37°C, avoid prolonged exposure the Buffer to the water bath.
2. Add 1 μL of SDS-PAGE Protein Loading Buffer (non-Reducing, 5×) to each 4 μL of protein sample, mix well.
3. Heat at 95°C or in a boiling water bath for 3-10 min.
4. Cool the mixture to room temperature, and add to the loading hole for electrophoresis.
5. The electrophoresis stops when the blue dye reaches near the bottom of the gel.
Storage
-20℃, 2 years
Attention
1. Dissolve completely before using the Buffer.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K1108-5 mL | HY-K1108-10 mL |
|---|---|---|
| SDS-PAGE Protein Loading Buffer (non-Reducing, 5×) | 1 mL × 5 | 1 mL × 10 |
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