2. Metabolic Enzyme/Protease Apoptosis
  3. Glutathione Peroxidase Ferroptosis
  4. RSL3

RSL3  (Synonyms: (1S,3R)-RSL3)

Cat. No.: HY-100218A Purity: 99.90%
COA Handling Instructions

RSL3 ((1S,3R)-RSL3) is an inhibitor of glutathione peroxidase 4 (GPX4) (ferroptosis activator), reduces the expression of GPX4 protein, and induces ferroptotic death of head and neck cancer cell. RSL3 increases the expression of p62 and Nrf2 and inactivates Keap1 in HN3-rslR cells.

For research use only. We do not sell to patients.

RSL3 Chemical Structure

RSL3 Chemical Structure

CAS No. : 1219810-16-8

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Solution
10 mM * 1 mL in DMSO USD 87 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 87 In-stock
Solid
2 mg USD 60 In-stock
5 mg USD 90 In-stock
10 mg USD 160 In-stock
25 mg USD 330 In-stock
50 mg USD 560 In-stock
100 mg USD 990 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 155 publication(s) in Google Scholar

RSL3 Related Antibodies

Top Publications Citing Use of Products

141 Publications Citing Use of MCE RSL3

Proliferation Assay
WB
IHC
IF

    RSL3 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are treated with OSMI-1 (30 μM) or TMG (10 μM) for 12 h, and then induced with or without RSL3 (10 μM) for 6 h.

    RSL3 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are treated with DMSO (Control) or OSMI-1 for 12 h, and then treated with RSL3 (10 μM) for different time as indicated. Cells are then subjected to immunofluorescence microscopy with antibodies against TfR1 and GM130.

    RSL3 purchased from MCE. Usage Cited in: Cell Discov. 2022 May 3;8(1):40.  [Abstract]

    U2OS cells are treated with DMSO (Control) or OSMI-1 for 12 h, and then treated with RSL3 (10 μM) for different time as indicated. The levels of cellular ferrous iron are assessed by flow cytometry using FerroOrange.

    RSL3 purchased from MCE. Usage Cited in: Nat Cancer. 2022 Apr;3(4):471-485.  [Abstract]

    Cellular responses of neuroblastoma cell lines to 72 h of RSL3 treatment: cells with MYCN amplification (black symbols), moderate MYCN expression (white circle) and lack thereof (white triangle).

    RSL3 purchased from MCE. Usage Cited in: Redox Biol. 2022 Jul 31;55:102408.  [Abstract]

    Immune blot detection of FSP1, GCLC, GCLM, and GPX4 in total protein extracts from the cell lines exposed to RSL3 or L-BSO for 24 h. The concentrations used are 0.25 and 1 μM RSL3 for CTV-1, Jurkat, K562 and HCT-116. For MOLT-16 RSL3 is used at 0.05 and 0.25 μM.

    RSL3 purchased from MCE. Usage Cited in: Int J Biol Sci. 2022 Jun 21;18(10):4135-4150.  [Abstract]

    CCK-8 assay assessed the cell viability of PANC1 and PaTU8988 cells treated DMSO, Erastin (4, 15 µM) and RSL3 (0.5, 5 µM) with or without SC-26196 (10 µM).

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 May 30;13(5):507.  [Abstract]

    HE staining of retinal tissue in mice that underwent sham or IR injury at 3 and 7 d after intravitreal injection of vehicle (DMSO) or 50 μM Erastin and 500 nM RSL-3.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):825.  [Abstract]

    MSCs are treated with Erastin (100 nmol/L) or RSL3 (10 nmol/L) or treated with Fer-1 (10 μmol/L) or Lip-1 (20 μmol/L) during SPIO labeling. Cells are harvested at 24 h. A Cell viability of MSCs under different treatment was measured by CCK-8 assay. Both of them reduce the cell viability of MSCs and induce the level of ROS and lipid peroxide in MSCs.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Sep 26;13(9):825.  [Abstract]

    MSCs are treated with Erastin (100 nmol/L) or RSL3 (10 nmol/L) or treated with Fer-1 (10 μmol/L) or Lip-1 (20 μmol/L) during SPIO labeling. Cells are harvested at 24 h.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2022 Jul 20;13(7):630.  [Abstract]

    ROS levels are detected in pancreatic cancer cells from different treatment groups with a ROS Assay Kit. MDA and GSH-PX levels are detected in RSL3 (3 μM; 24 h) of treated Panc-1, MIA PaCa-2, and BxPC-3 cells.

    RSL3 purchased from MCE. Usage Cited in: Redox Biol. 2021 Nov;47:102174.  [Abstract]

    HT1080 fibrosarcoma cells are exposed to RSL3 (10 μM, 24 h), and phorbol myristate acetate (PMA)-primed THP1 macrophage cells were exposed to crocidolite in the presence of absence of ferric ammonium citrate (Fe, 100 μg/ml) up to 96 h. Membrane-catch transmission electronmicroscopy (TEM) images of EVs on carbon grids.

    RSL3 purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Nov 13;12(11):1079.  [Abstract]

    The protein levels of Keap1, Nrf2 and HO-1 in HCT116 and DLD-1 cells are measured by western blotting after treatment with RSL3 (1 μM), cetuximab (100 μg/ml) or their combination for 24 h.

    RSL3 purchased from MCE. Usage Cited in: Brain Res Bull. 2020 Oct;163:40-48.  [Abstract]

    Gpx4 and 4-HNE expression assessment by Western blotting after RSL3 treatment. Western blot analysis reveal that compared with the Mel group, the RSL3 group has significantly decreased and increased Gpx4 and 4-HNE expression, respectively.

    RSL3 purchased from MCE. Usage Cited in: Brain Res Bull. 2020 Oct;163:40-48.  [Abstract]

    Representative images and NeuN + cell quantification in the hippocampus via immunohistochemistry after RSL3 treatment. Compared with the Mel group, the RSL3 group has a significantly decreased NueN + cell number.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    RSL3 ((1S,3R)-RSL3) is an inhibitor of glutathione peroxidase 4 (GPX4) (ferroptosis activator), reduces the expression of GPX4 protein, and induces ferroptotic death of head and neck cancer cell. RSL3 increases the expression of p62 and Nrf2 and inactivates Keap1 in HN3-rslR cells[1].

    IC50 & Target

    Glutathione peroxidase 4[1]
    In Vitro

    RSL3 (0-8 μM, 72 hours) potently reduces the viability of HN3 cells, with IC50s of 0.48 μM in HN3 and 5.8 μM in HN3-rslR cells, respectively[1].
    RSL3 (0-8 μM, 24 hours) reduces the expression of GPX4 protein, increases the expression of p62 and Nrf2 and inactivates Keap1 in HN3-rslR cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    RSL3 Related Antibodies

    Cell Viability Assay[1]

    Cell Line: HN3 cells, HN3-rslR cells
    Concentration: 0-8 μM
    Incubation Time: 72 hours
    Result: Showed IC50s of 0.48 µM in HN3 and 5.8 µM in HN3-rslR cells, respectively[1].

    Western Blot Analysis[1]

    Cell Line: HN3-rslR cells
    Concentration: 0-8 μM
    Incubation Time: 24 hours
    Result: Inhibited GPX4 expression, increased p62 and Nrf2 levels, and decreased Keap1 levels.
    In Vivo

    RSL3 (100 mg/kg, Intratumorally twice per week for 20 days) significantly inhibits the growth of tumor in combination with Trigonelline (HY-N0414) in mice bearing HN3R cells[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Ten-week-old athymic BALB/c male nude mice (nu/nu) bearing HN3R cells[1]
    Dosage: 100 mg/kg in combination with trigonelline (50 mg/kg)
    Administration: Intratumorally twice per week for 20 days
    Result: Significantly reduced the volume of tumor combined with trigonelline in mice.
    Molecular Weight

    440.88

    Appearance

    Solid

    Formula

    C23H21ClN2O5
    CAS No.
    SMILES

    O=C([C@H]1CC2=C([C@H](C3=CC=C(C(OC)=O)C=C3)N1C(CCl)=O)NC4=C2C=CC=C4)OC
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (226.82 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2682 mL 11.3410 mL 22.6819 mL
    5 mM 0.4536 mL 2.2682 mL 4.5364 mL
    10 mM 0.2268 mL 1.1341 mL 2.2682 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  50% PEG300    50% saline

      Solubility: 20 mg/mL (45.36 mM); Suspended solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: 5 mg/mL (11.34 mM); Suspended solution; Need ultrasonic

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (5.67 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (4.72 mM); Clear solution

    • 5.

      Add each solvent one by one:  10% DMF    90% corn oil

      Solubility: ≥ 0.56 mg/mL (1.27 mM); Clear solution

    *All of the co-solvents are available by MCE.
    Purity & Documentation

    Purity: 99.90%

    COA (262 KB) HNMR (310 KB) RP-HPLC (244 KB) LCMS (94 KB) Elemental Analysis Report (120 KB)

    Handling Instructions (2659 KB)
    References
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    RSL3 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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    × = ×
    C1   V1   C2   V2

    Keywords:

    RSL31219810-16-8(1S,3R)-RSL3RSL 3RSL-3Glutathione PeroxidaseFerroptosisInhibitorinhibitorinhibit

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