1. Biochemical Assay Reagents
  2. DTT

DTT 

Cat. No.: HY-15917 Purity: 98.71%
Handling Instructions

DTT is a reducing agent. DTT forms a stable six-membered ring with an internal disulfide bond once oxidized.

For research use only. We do not sell to patients.

DTT Chemical Structure

DTT Chemical Structure

CAS No. : 3483-12-3

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 102 In-stock
Estimated Time of Arrival: December 31
10 g   Get quote  
50 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

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Description

DTT is a reducing agent. DTT forms a stable six-membered ring with an internal disulfide bond once oxidized.

In Vitro

DTT is widely used in biochemistry works to reduce dissulfide bridges, protect biomolecules, in sample preparation, and to denature proteins before electrophoresis analysis (SDS-PAGE).

Molecular Weight

154.25

Formula

C₄H₁₀O₂S₂

CAS No.

3483-12-3

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : ≥ 200 mg/mL (1296.60 mM)

DMSO : 125 mg/mL (810.37 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 6.4830 mL 32.4149 mL 64.8298 mL
5 mM 1.2966 mL 6.4830 mL 12.9660 mL
10 mM 0.6483 mL 3.2415 mL 6.4830 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (13.48 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (13.48 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (13.48 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: 98.71%

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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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DTTOthersInhibitorinhibitorinhibit

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DTT
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HY-15917
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