Human iPSC-Derived Brain Organoid Long-term Culture Medium
MCE Human iPSC-Derived Brain Organoid Long-term Culture Medium is a specialized culture system developed through systematic reformulation and concentration optimization based on neuronal cell culture platforms. It significantly enhances the adaptability and stability of long-term culture for brain organoids derived from human induced pluripotent stem cells (iPSCs).
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Storage :
-20°C, 2 years.
- Manual (2288KB)
- COA
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Description & Advantages
MCE Human iPSC-Derived Brain Organoid Long-term Culture Medium is a specialized culture system developed through systematic reformulation and concentration optimization based on neuronal cell culture platforms. It significantly enhances the adaptability and stability of long-term culture for brain organoids derived from human induced pluripotent stem cells (iPSCs).
By optimizing neurotrophic support, this medium promotes neuronal differentiation, maturation, and synaptic network formation. In addition, it incorporates multiple antioxidant and stress-modulating components to effectively alleviate oxidative stress and cell death commonly observed during prolonged brain organoid culture, thereby markedly improving organoid viability, structural integrity, and culture consistency.
This product is provided as a ready-to-use basal medium and is supplemented with neuron-specific additives (Bi-27 and N-2), achieving a balanced regulation of neuronal proliferation and differentiation. It provides a stable and reliable culture environment for long-term maintenance of brain organoids (> 90 d).
Protocol
Reagents and Consumables Required
DPBS (HY-K3008), Basement Membrane Matrix GFR (HY-K6004), Basement Membrane Matrix IPSC-qualified (HY-K6006), Organoid Dissociation Solution (HY-K6013), iPSC/ESC Dissociation Solution (Enzyme-Free) (HY-K6020), CEPT Cocktail Plus (1000×) (HY-K6021), Human iPSC/ESC Whole-Brain Organoid Induction Differentiation Kit (HY-K6302), Human PSC Embryoid Body (EB) Induction Medium (HY-K6503), horizontal centrifugation equipment, low-attachment 6-well plates, and related consumables.
Organoid Morphological Evaluation Criteria
When EBs reach approximately 1.0-1.2 mm in diameter, evaluate them by bright-field microscopy. A dense SOX2+/PAX6+ neuroepithelial core should be visible centrally, surrounded by a more loosely organized, radially polarized N-Cadherin+/ZO-1+ neuroectodermal layer. Clear formation of these features indicates entry into the neural maturation phase.
Mechanical Dissection of Organoids
1. Under sterile conditions, transfer EBs to pre-chilled DPBS at 4°C and incubate for 5 min to reduce matrix adhesiveness.
2. Using a 27G syringe with the bevel facing outward, make controlled circumferential cuts along the peripheral glial layer (GFAP+ region) to gradually remove the outer fibrotic matrix.
3. Immediately transfer the dissected EBs into a low-attachment 6-well plate containing 3 mL Human iPSC-Derived Brain Organoid Long-term Culture Medium per well.
Adjustment of Dynamic Culture Parameters
1. Increase orbital shaker speed to 120 rpm to improve mass transfer at the medium-tissue interface and reduce the risk of central hypoxia.
2. Perform a complete medium replacement every 3 d to limit metabolic-waste accumulation and maintain a stable long-term culture environment.
Storage
-20°C, 2 years.
Attention
1. This product is sterile and should be handled using aseptic techniques. It is recommended to aliquot and store to avoid contamination.
2. This product is for R&D use only, not for drug, household, or other uses.
3. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K6502-100 mL |
|---|---|
| Human iPSC-Derived Brain Organoid Long-term Culture Medium | 100 mL |
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