1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis

DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis Related Products (38):

Cat. No. Product Name Effect Purity
  • HY-12320
    Cycloheximide Inhibitor 99.17%
    Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, with IC50 of 532.5 nM and 2880 nM for protein synthesis and RNA synthesis in vivo, respectively.
  • HY-17559
    Actinomycin D Inhibitor 99.89%
    Actinomycin D inhibits DNA repair with IC50 of 0.42 μM.
  • HY-13316
    Mitomycin C Inhibitor 98.51%
    Mitomycin C is a DNA-damaging agent and small-molecule inhibitor effectively sensitize cancer cells to tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL).
  • HY-17026
    Gemcitabine Inhibitor >98.0%
    Gemcitabine is a DNA synthesis inhibitor with IC50 of 37.6, 42.9, 92.7, 89.3 and 131.4 nM in BxPC-3, Mia Paca-2, PANC-1, PL-45 and AsPC-1 cells, respectively.
  • HY-12742
    SCR7 Inhibitor 99.78%
    SCR7 is a DNA Ligase IV inhibitor, blocks nonhomologous end-joining (NHEJ).
  • HY-U00337
    Datelliptium chloride Inhibitor
    Datelliptium chloride is a DNA-intercalating agent derived from ellipticine, with anti-tumor activities.
  • HY-13753
    Streptozocin Inhibitor 99.58%
    Streptozocin is a potent DNA-methylating agent, with IC50 values of 11.7, 904 and 1024 μg/mL for HL60, K562 and C1498 cells respectively.
  • HY-19610
    alpha-Amanitin Inhibitor
    alpha-Amanitin is the principal toxin of several deadly poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II.
  • HY-B0003
    Gemcitabine Hydrochloride Inhibitor 99.62%
    Gemcitabine hydrochloride is a DNA synthesis inhibitor with IC50 of 37.6, 42.9, 92.7, 89.3 and 131.4 nM in BxPC-3, Mia Paca-2, PANC-1, PL-45 and AsPC-1 cells, respectively.
  • HY-17565
    Bleomycin sulfate Inhibitor >98.0%
    Bleomycin sulfate is a potent DNA damaging agent, as the best-studied micronucleus (MN) inducer.
  • HY-B0016
    Capecitabine Inhibitor 99.68%
    Capecitabine is an oral prodrug that is converted to its only active metabolite, fluorouracil (FU), by thymidine phosphorylase.
  • HY-13323
    CX-5461 Inhibitor 98.47%
    CX-5461 is an inhibitor of rRNA synthesis, and selectively inhibits Pol I-driven transcription of rRNA with IC50 of 142 nM in HCT-116, A375, and MIA PaCa-2 cells, with no effect on Pol II, and possesses 250- to 300-fold selectivity for inhibition of rRNA transcription versus DNA replication and protein translation.
  • HY-15457
    Triciribine Inhibitor 99.20%
    Triciribine is a DNA synthesis inhibitor, also inhibits Akt and HIV-1/2 with IC50 of 130 nM, and 0.02-0.46 μM, respectively.
  • HY-B0313
    Hydroxyurea Inhibitor >98.0%
    Hydroxyurea is a cell apoptosis inducer that inhibitDNA synthesis through inhibition of ribonucleotide reductase.
  • HY-19620
    LMI070 Agonist 98.78%
    LMI070 (NVS-SM1) is a highly potent, selective and orally active small molecule SMN2 splicing modulator.
  • HY-19931
    COH29 Inhibitor 98.04%
    COH29 is a potent ribonucleotide reductase (RNR) inhibitor with anticancer activity with an IC50 of 8 μM in KB cell.
  • HY-16637
    Folic acid >98.0%
    Folic acid(Vitamin M; Vitamin B9) is a B vitamin; is necessary for the production and maintenance of new cells, for DNA synthesis and RNA synthesis.
  • HY-10082
    Triapine Inhibitor 98.32%
    Triapine is a novel inhibitor of the M2 subunit of ribonucleotide reductase (RR), and is a potent radiosensitizer.
  • HY-15586
    L67 Inhibitor >98.0%
    L67 is a novel, competitive human DNA ligase inhibitor, inhibits DNA ligases I and III with IC50 of 10 μM and 10 μM.
  • HY-12814
    TH588 Inhibitor 98.52%
    TH588 is first-in-class nudix hydrolase family inhibitor that potently and selectively engage and inhibit the MTH1(IC50= 5 nM) in cells.