1. Others
  2. Fluorescent Dye
  3. Propidium Iodide

Propidium Iodide 

Cat. No.: HY-D0815 Purity: 99.50%
COA Handling Instructions

Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

For research use only. We do not sell to patients.

Propidium Iodide Chemical Structure

Propidium Iodide Chemical Structure

CAS No. : 25535-16-4

Size Price Stock Quantity
Free Sample (0.1 - 0.5 mg)   Apply Now  
5 mg USD 32 In-stock
10 mg USD 50 In-stock
50 mg USD 99 In-stock
100 mg USD 170 In-stock
500 mg USD 580 In-stock
1 g   Get quote  
5 g   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 44 publication(s) in Google Scholar

Top Publications Citing Use of Products

36 Publications Citing Use of MCE Propidium Iodide

IF
Proliferation Assay

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Feb 8.  [Abstract]

    Propidium iodide (PI) staining for cell death with GCFN and ferroptosis inhibitor ferrostatin-1 treatment for 48 h

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Apr 5;920:174830.  [Abstract]

    Primary newborn rat cardiac fibroblasts are stained with PI (2 μg/mL; for 2 h) and analyzed using a Nikon Ti2 microscope.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Oct 9;27(19):6733.  [Abstract]

    In A549 cells, Propidium iodide (PI, 50 μg/mL) is added for incubation for 30 min in the dark. Cell-cycle distribution is determined using a FACSaria-I flow cytometer.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2022 May;13(9):1299-1310.  [Abstract]

    KYSE450 and ECA109 cells after 96 h various transfections were washed three times in cold PBS, stained with Propidium iodide (PI, 50 μg/mL) and Annexin V‐FITC (25 μg/mL) for 20 min in the dark, and analyzed within 1 h.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207.  [Abstract]

    PAMs and PK-15CD163 cells sre washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei sre counterstained with propidium iodide (red). Fluorescent images are acquired with a confocal laser scanning microscope.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Aug 31;2021:5608133.

    The NP tissue slices are incubated with a culture medium containing 1 μg/ml Calcein AM and 1 μg/ml PI for one hour at 37°C. After washing with PBS, the slices are immediately viewed using an inverted confocal laser scanning microscope.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Nov 28;10(11):3353-3370.  [Abstract]

    KLE cells are stained by 25 μg/mL PI in fluorescence-activated cell sorting buffer, incubated cells for 30 min in dark environment at room temperature. Cell apoptosis is determined by Annexin V/PI double staining and percentages of cells in each phase are calculated.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.

    PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

    In Vitro

    "General Protocol

    1.Preparation of PI working solution
    1.1 Preparation of the stock solution
    Dissolve 1 mg PI in 1 mL DDH2O to obtain 1 mg/mL of stock solution.
    Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
    1.2 Preparation of PI working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 20-50 μg/mL of working solution.
    Note: Please adjust the concentration of PI working solution according to the actual situation.
    2.Cell staining
    2.1 Suspension cells(6-well plate)
    a.Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×106/mL.
    b.Add 1 mL of working solution, and then incubate at room temperature for 5-10 minutes.
    c.Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    d.Wash twice with PBS, 5 minutes each time.
    e.Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    2.2 Adherent cells
    a. Culture adherent cells on sterile coverslips.
    b. Remove the coverslip from the medium and aspirate excess medium.
    c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-10 minutes.
    d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

    Storage
    -20°C, 1 year
    Protect from light

    Precautions
    1. Please adjust the concentration of PI working solution according to the actual situation.
    2. This product is for R&D use only, not for drug, household, or other uses.
    3. For your safety and health, please wear a lab coat and disposable gloves to operate.
    "

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    668.39

    Formula

    C27H34I2N4

    CAS No.
    Appearance

    Solid

    Emission (Em)

    615

    Excitation (Ex)

    535

    SMILES

    C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (149.61 mM; Need ultrasonic)

    H2O : 3.57 mg/mL (5.34 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4961 mL 7.4807 mL 14.9613 mL
    5 mM 0.2992 mL 1.4961 mL 2.9923 mL
    10 mM 0.1496 mL 0.7481 mL 1.4961 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.74 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.74 mM); Clear solution

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.68%

    Dyeing Example
    References
    Cell Assay
    [2]

    Flow cytometric analysis: Propidium iodide is prepared in in 0.1% sodium citrate plus 0.1% Triton X-100 (50 μg/mL). The 200 ×g centrifuged cell pellet is gently resuspended in 1.5 mL hypotonlc fluorochrome solution (Propidium iodide 50 μg/mL), in 12×75 polypropylene tubes. The tubes are placed at 4°C in the dark overnight before the flow cytometric analysis. The propidium Iodide fluorescence of individual nuclei is measured using a FACScan flow cytometer. The nuclei traverses the light beam of a 488 nm Argon laser. A 560 nm dichrolc nurror (DM 570) and a 600 nm band pass filter (bandwidth 35 nm) are used for collecting the red fluorescence due to propidium Iodide staining of DNA and the data are registered on a logarithmic scale[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Propidium Iodide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

     

    Salutation

    Applicant Name *

     

    Email Address *

    Phone Number *

     

    Organization Name *

    Department *

     

    Requested quantity *

    Country or Region *

         

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Propidium Iodide
    Cat. No.:
    HY-D0815
    Quantity:
    MCE Japan Authorized Agent: