1. Dye Reagents
  2. Propidium Iodide

Propidium Iodide (Synonyms: PI)

Cat. No.: HY-D0815 Purity: 99.68%
Handling Instructions

Propidium Iodide is a red-fluorescent dye that can be used to stain cells.

For research use only. We do not sell to patients.

Propidium Iodide Chemical Structure

Propidium Iodide Chemical Structure

CAS No. : 25535-16-4

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10 mg USD 50 In-stock
Estimated Time of Arrival: December 31
50 mg USD 99 In-stock
Estimated Time of Arrival: December 31
100 mg USD 170 In-stock
Estimated Time of Arrival: December 31
500 mg USD 580 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 8 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Propidium Iodide purchased from MCE. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.

    PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.
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    Propidium Iodide is a red-fluorescent dye that can be used to stain cells.

    In Vitro

    Propidium Iodide is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5 base pairs with little sequence preference. Propidium Iodide has evidenced of having no toxic effects on neurons, being today’s most common marker for membrane integrity and cell viability when applied prior to fixation (pre-fixation Propidium Iodide staining method). The pre-fixation staining has been widely used for quantitative assessments of neuronal cell decline in models of acute neurodegeneration, visualized as intensely labeled PI+-pycnotic nuclei of degenerating neurons [1]. Propidium Iodide cannot cross the membrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometric analysis. The flow cytometric data shows an excellent correlation with the results obtained with both electrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.





    Room temperature in continental US; may vary elsewhere.


    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    H2O : 3.57 mg/mL (5.34 mM; ultrasonic and warming and heat to 60°C)

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4961 mL 7.4807 mL 14.9613 mL
    5 mM 0.2992 mL 1.4961 mL 2.9923 mL
    10 mM --- --- ---
    *Please refer to the solubility information to select the appropriate solvent.
    Cell Assay

    Flow cytometric analysis: Propidium iodide is prepared in in 0.1% sodium citrate plus 0.1% Triton X-100 (50 μg/mL). The 200 ×g centrifuged cell pellet is gently resuspended in 1.5 mL hypotonlc fluorochrome solution (Propidium iodide 50 μg/mL), in 12×75 polypropylene tubes. The tubes are placed at 4°C in the dark overnight before the flow cytometric analysis. The propidium Iodide fluorescence of individual nuclei is measured using a FACScan flow cytometer. The nuclei traverses the light beam of a 488 nm Argon laser. A 560 nm dichrolc nurror (DM 570) and a 600 nm band pass filter (bandwidth 35 nm) are used for collecting the red fluorescence due to propidium Iodide staining of DNA and the data are registered on a logarithmic scale[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.


    Purity: 99.68%

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