Live-cell imaging and analysis of 3D spheroids in hypoxia- and radiotherapy-related research

  • Clin Transl Radiat Oncol. 2025 Jan 15:51:100920. doi: 10.1016/j.ctro.2025.100920.
Claire Beckers  1 ,  Lazaros Vasilikos  1 ,  Lorena Moor  1 ,  Martin Pruschy  1
Affiliations
  • 1. Laboratory for Applied Radiobiology, Department of Radiation Oncology, University Hospital Zurich, University of Zurich, Zurich, Switzerland.
PMID: 39898333 DOI: 10.1016/j.ctro.2025.100920
Abstract

3D spheroids are a valuable tool for investigating the interplay between hypoxia and radioresistance. However, standardized methods for visualizing normoxic and hypoxic regions within spheroids and assessing treatment responses are lacking. We developed a straightforward workflow for the analysis of spheroids based on image processing using a novel custom-written script, and flow cytometry.

Keywords
3D cell culture; Flow cytometry; Fluorescence microscopy; Hypoxia; Radiotherapy; Spheroids.
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