2. MCE Kits
  3. Molecular Biology
  4. RT-PCR
  5. Reverse transcription PCR
  6. RT Master Mix for qPCR Ⅲ (poly A)

RT Master Mix for qPCR Ⅲ (poly A) 

Cat. No.: HY-K0512
Manual SDS

MCE RT Master Mix for qPCR Ⅲ (poly A) uses poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA.

RT Master Mix for qPCR Ⅲ (poly A)
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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MicroRNAs are a class of non-coding RNAs with a length of about 22 nt, which play an important role in the regulation of gene expression in plants and animals.

MCE RT Master Mix for qPCR Ⅲ (poly A) uses poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA. The kit contains poly(A) polymerase (PAP) for the miRNA 3' end poly(A) reaction and all the raw materials and primers for miRNA reverse transcription reaction. It ensures that poly(A) modification process and reverse transcription process can be performed simultaneously and efficiently in miRNA 3' end. Only design miRNA-specific forward primers and use them together with the universal reverse primers included in the kit for subsequent qPCR experiments. Additionally, this kit provides universal U6 internal forward/reverse primers applicable for human, rat, and mouse that can be used for plotting standard curves.
Storage

-20ºC, 1 year

Avoid repetitive freeze-thaw cycles
Components
Components HY-K0512-10 rxns HY-K0512-50 rxns
miRNA RT Mix 17.5 μL 87.5 μL
2 × miRNA RT Buffer 50 μL 250 μL
Universal Reverse Primer (10 μM) 800 μL 4 mL
U6 Forward Primer (10 μM) 100 μL 500 μL
U6 Reverse Primer (10 μM) 100 μL 500 μL
RNase-Free H2O 2 mL 10 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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RT Master Mix for qPCR Ⅲ (poly A)
Cat. No.:
HY-K0512
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