microRNA RT Master Mix for qPCR Ⅲ (poly A) 

MicroRNAs are a class of non-coding RNAs with a length of about 22 nt, which play an important role in the regulation of gene expression in plants and animals.

MCE microRNA RT Master Mix for qPCR Ⅲ (poly A) uses poly(A)-tailing method to perform reverse transcription from miRNA first-strand to cDNA. The kit contains poly(A) polymerase (PAP) for the miRNA 3' end poly(A) reaction and all the raw materials and primers for miRNA reverse transcription reaction. It ensures that poly(A) modification process and reverse transcription process can be performed simultaneously and efficiently in miRNA 3' end. Only design miRNA-specific forward primers and use them together with the universal reverse primers included in the kit for subsequent qPCR experiments. Additionally, this kit provides universal U6 internal forward/reverse primers applicable for human, rat, and mouse that can be used for plotting standard curves.

-20°C, 2 years

Avoid repetitive freeze-thaw cycles