Human iPSC/ESC Cardiomyocyte Induction Differentiation Kit 

MCE Human iPSC/ESC Cardiomyocyte Induction Differentiation Kit is based on the classical GiWi system. By precisely modulating the Wnt/β-catenin signaling pathway in a temporally controlled manner (sequential activation and inhibition), the kit enables highly efficient directed differentiation of human pluripotent stem cells into cardiomyocytes. This system recapitulates key stages of cardiac development:

 Wnt activation phase (≈ 2 d): Induces specification of the cardiogenic lateral plate mesoderm and promotes progenitor cell expansion.

 Wnt inhibition phase (≈ 4 d): Drives cardiomyocyte progenitors toward contractile cardiomyocyte lineages.

 Maturation phase (≈ 8 d): Combined with metabolic selection (glucose-free, high-lactate culture), promotes sarcomere assembly, electrophysiological maturation, and metabolic remodeling (e.g., the MYH6-to-MYH7 expression shift).

Through this workflow, synchronized spontaneously beating, high-purity (cTnT⁺), and electrophysiologically mature induced cardiomyocytes (iCMs) can be obtained within 14-15 d.

The kit provides a chemically defined medium system covering all five essential stages of differentiation, supporting initiation from a 24-well plate scale and allowing maintenance of cultures up to day 20. This system offers high reproducibility and robust functional performance, making it suitable for cardiac disease modeling, drug cardiotoxicity assessment, and mechanistic studies.

-20°C, 2 years.

1. This product is sterile and should be handled using aseptic techniques. It is recommended to aliquot and store to avoid contamination.

2. This product is for R&D use only, not for drug, household, or other uses.

3. For your safety and health, please wear a lab coat and disposable gloves to operate.