RT Master Mix for qPCR II

Cat. No.: HY-K0510A: FAQs
Manual SDS

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. With advanced reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically.

Size	Price	Stock	Quantity
Free Sample (10 rxns)		Apply Now
100 rxns	USD 210	In-stock	Estimated Time of Arrival: December 31
500 rxns	USD 940	In-stock	Estimated Time of Arrival: December 31

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RT Master Mix for qPCR II Featured Recommendations:

Description
Storage
Protocol
Components
Documentation

Description
& Advantages

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. The 4× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis. With RNA added, the optimized system will provide sensitive and reliable cDNA synthesis. In addition, the increased heat resistance of the advanced reverse transcriptase allows reverse transcription to be performed at a higher temperature, increasing the efficiency and specificity of cDNA synthesis.

Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommened for detection of the expression levels of interested genes.

Features:

1. Synthesize cDNA rapidly and specifically.

2. Only one transfer step reduces handling errors significantly.

3. Optimized buffer enhances sensitive and reliable cDNA synthesis.

4. Super-efficient reaction even with low template amounts (total RNA: 50 pg -5 μg in a 20 μL reaction).

5. The kit provides both Oligo dT Primer and Random Primer.

Publications

Storage

Stored at -20°C, and is stable for up to 18 months.

Avoid repetitive freeze-thaw cycles.

Protocol

1 Thaw RNA templates and the 4× Super RT Mix on ice. Mix solutions gently but thoroughly.

2 Prepare the following reaction mixture in a PCR tube on ice.

Components	Quantity
4× Super RT Mix	5 μL
Total RNA / mRNA	50 pg-5 μg / 50 pg-500 ng
RNase-Free H₂O	To 20 μL

3 Mix the components well and collect by brief centrifugation. Incubate the mixture in a PCR instrument or water bath as follows:

Temperature	Time
25ºC	5 mins
55ºC	15 mins
85ºC	2 mins

Note:

a. For GC rich or structurally complex RNA templates, increasing the RT incubation temperature up to 60ºC may improve the yields of cDNA.

b. Stop the reaction by heating at 85ºC for 2 mins followed by chilling on ice.

4 The newly synthesized first-strand cDNA is ready for immediate downstream applications or for long-term storage at -20ºC.

Components

Contents	HY-K0510A-100 rxns	HY-K0510A-500 rxns
4× Super RT Mix	500 μL	500 μL × 5
RNase-Free H₂O	1 mL × 2	1 mL × 10

Documentation

Manual (239 KB) SDS (171 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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