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RT Master Mix for qPCR II

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MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. With advanced reverse transcriptase, this kit can synthesize cDNA more rapidly, more specifically. The 100 rxns is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :

    -20°C, 2 years

    Avoid repetitive freeze-thaw cycles

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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

MCE RT Master Mix for qPCR II is a convenient, ready-to-use formulation for reverse transcription. The 4× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis. With RNA added, the optimized system will provide sensitive and reliable cDNA synthesis. In addition, the increased heat resistance of the advanced reverse transcriptase allows reverse transcription to be performed at a higher temperature, increasing the efficiency and specificity of cDNA synthesis.

Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommened for detection of the expression levels of interested genes.

 

Features:

1. Synthesize cDNA rapidly and specifically.

2. Only one transfer step reduces handling errors significantly.

3. Optimized buffer enhances sensitive and reliable cDNA synthesis.

4. Super-efficient reaction even with low template amounts (total RNA: 50 pg -5 μg in a 20 μL reaction).

5. The kit provides both Oligo dT Primer and Random Primer.

Protocol

1. Thaw RNA templates and the 4× Super RT Mix on ice. Mix solutions gently but thoroughly.

2. Prepare the following reaction mixture in a PCR tube on ice.

Components Quantity
4× Super RT Mix 5 μL
Total RNA / mRNA 50 pg-5 μg / 50 pg-500 ng
RNase-Free H2O To 20 μL

3. Mix the components well and collect by brief centrifugation. Incubate the mixture in a PCR instrument or water bath as follows:

Temperature Time
25°C 5 min
55°C 15 min
85°C 2 min

Note:

a. For GC rich or structurally complex RNA templates, increasing the RT incubation temperature up to 60°C may improve the yields of cDNA.

b. Stop the reaction by heating at 85°C for 2 min followed by chilling on ice.

4. The newly synthesized first-strand cDNA is ready for immediate downstream applications or for long-term storage at -20°C.

Storage

-20°C, 2 years

Avoid repetitive freeze-thaw cycles

Components

Contents HY-K0510A-100 rxns HY-K0510A-500 rxns
4× Super RT Mix 500 μL 500 μL × 5
RNase-Free H2O 1 mL × 2 1 mL × 10

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100 mg

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