Fluorescein Phalloidin
Based on 6 publication(s) in Google Scholar
MCE Fluorescein Phalloidin is Phalloidin conjugated to the fluorescent dye Fluorescein. Phalloidin binds F-actins with high selectivity while Fluorescein provides stable and bright green fluorescence.
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Storage :
The solutions should be stable for at least 6 months if store at -20°C.
Protecting from light, and avoid freeze/thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Phalloidin is a mushroom-derived toxin which can be used to specifically label F-actins (actin filaments) of the cytoskeleton. It binds to all variants of actin filaments in many different species of animals and plants. Typically, Phalloidin is used conjugated to a fluorescent dye. Phalloidin binds F-actins with high selectivity while Fluorescein provides stable and bright green fluorescence.
Fluorescein Phalloidin is 1000× stock solution in DMSO which is convenient for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
Protocol
1. Prepare 1× Fluorescein Phalloidin working solution:
Add 1 μL of 1000× Fluorescein Phalloidin DMSO solution to 1 mL of PBS containing with 1% BSA.
Note: a. Different cell types might be stained differently. The concentration of Fluorescein Phalloidin working solution should be prepared accordingly.
b. 1% bovine serum albumin (BSA) is used to reduce nonspecific background staining.
2. Stain the cells:
2.1 Wash cells 2-3 times with PBS. Fix the cells in 3.7% methanol-free formaldehyde solution in PBS for 10-30 minutes at room temperature.
Note: Avoid any methanol containing fixatives since methanol can disrupt actin during the fixation process. The preferred fixative is methanol-free formaldehyde.
2.2 Wash the fixed cells 2-3 times in PBS.
2.3 Add 0.1% Triton X-100 in PBS into fixed cells for 3-5 minutes to increase permeability. Wash the cells 2-3 times in PBS.
2.4 Add 100 μL/well (96-well plate) of Fluorescein Phalloidin working solution into the fixed cells, and stain the cells at room temperature for 20 to 90 minutes.
2.5 Rinse cells gently with PBS 2-3 times to remove excess AMCA Phalloidin.
2.6 Run fluorescence microscope or other equipments at desired Ex/Em wavelengths (AMCA Phalloidin, Ex/Em=344/432 nm).
Publications
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Journal Impact Factor
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Most Recent
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Mater Today BioImmuno-protective cationic ginsenoside Rb1 vesicles for macrophage-mediated targeted therapy of inflammatory diseases. [Abstract]2025 Aug 12:34:102195. PMID: 40893350
Fluorescein Phalloidin purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Aug 12:34:102195. [Abstract]
Confocal microscopy images showing the cellular uptake of nanosized Rb1 in RAW 264.7 cells. F-actin was stained with phalloidin (1:1000 in PBS, 1 h, room temperature, in the dark).
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OncogeneRetrograde regulation of mitochondrial fission and epithelial to mesenchymal transition in hepatocellular carcinoma by GCN5L1. [Abstract]2023 Mar;42(13):1024-1037. PMID: 36759571
Fluorescein Phalloidin purchased from MedChemExpress. Usage Cited in: Oncogene. 2023 Mar;42(13):1024-1037. [Abstract]
Morphology of HepG2 cells with or without GCN5L1 deletion, assessed by phalloidin staining (1:1000 dilution).
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J EthnopharmacolCompound kushen injection inhibits breast cancer lung metastasis through regulating MTSS1/ARPC3/F-actin. [Abstract]2025 Jun 26:350:120054. PMID: 40446975
Fluorescein Phalloidin purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2025 Jun 26:350:120054. [Abstract]
Immunofluorescence imaging of F-actin using phalloidin staining in 4T1 and MDA-MB-231 cells treated with MT (50 μM, 24 h). Phalloidin was diluted in PBS containing 5% bovine serum albumin and incubated at room temperature for 1 h in the dark.
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J Med VirolHPV16 Enhances Radiosensitivity in Esophageal Squamous Cell Carcinoma by Promoting De Novo Fatty Acid Synthesis and Disulfidptosis. [Abstract]2025 Nov;97(11):e70699. PMID: 41231234
Fluorescein Phalloidin purchased from MedChemExpress. Usage Cited in: J Med Virol. 2025 Nov;97(11):e70699. [Abstract]
Representative fluorescent staining images of F-actin. Phalloidin-FITC staining was performed for 30 min at room temperature in the dark. Images show LV-vector and LV-E6/E7 TE-1 cells under a negative control or 2 Gy IR treatment.
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Hum CellCollagen alpha-5(IV) chain activation by nuclear factor 1/C promotes nasopharyngeal carcinoma progression. [Abstract]2025 Aug 20;38(5):148. PMID: 40833521
Fluorescein Phalloidin purchased from MedChemExpress. Usage Cited in: Hum Cell. 2025 Aug 20;38(5):148. [Abstract]
The intensity and distribution of F-actin in NPC cells were examined using phalloidin (5 μg/mL, 40 min at 37 °C) staining.
Storage
The solutions should be stable for at least 6 months if store at -20°C.
Protecting from light, and avoid freeze/thaw cycles.
Components
| Cat. No. | Product Name | Ex (nm) | Em (nm) | Size | Package |
|---|---|---|---|---|---|
| HY-K0902 | Fluorescein Phalloidin | 497.0 | 516.0 | 300 T | 30 μL |
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