1. MCE Kits
  2. Protein Biology
  3. Protein Purification Protein Electrophoresis & WB
  4. Binding/Washing Buffer
  5. PBS-T Powder (1 L of 1×)

PBS-T Powder (1 L of 1×) 

Cat. No.: HY-K1022
Manual SDS

MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20, and is widely used in ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

PBS-T Powder (1 L of 1×)
Size Price Stock Quantity
Free Sample (2 pouches)   Apply Now  
20 pouches USD 32 In-stock
Estimated Time of Arrival: December 31
100 pouches USD 95 In-stock
Estimated Time of Arrival: December 31

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

PBS-T buffer is widely used as washing buffer in immunoassay experiments such as ELISA and Western Blot, and can also be used for antibody dilution and preparation of blocking solution.

MCE PBS-T Powder (1 L of 1×) consists of NaCl, KCl, Phosphate and Tween 20. Each pouch can be diluted to 1 L 1× PBS-T buffer, in which the concentration of NaCl is 137 mM, the concentration of KCl is 2.7 mM, the concentration of Phosphate is 10 mM and the concentration of Tween 20 is 0.05%. This product provides a convenient way to make PBS-T buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1.   Add 1 pouch of PBS-T Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2.   Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× PBS-T buffer.

Note: The pH of the 1× PBS-T buffer is 7.4 ± 0.2 @ 25°C.

Components
Components HY-K1022-20 pouches HY-K1022-100 pouches
PBS-T Powder (1 L of 1×) 20 pouches 5 × 20 pouches
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
PBS-T Powder (1 L of 1×)
Cat. No.:
HY-K1022
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