1. MCE Kits
  2. Protein Biology
  3. Protein Electrophoresis & WB
  4. Transfer Buffer
  5. Tris-Glycine Powder

Tris-Glycine Powder 

Cat. No.: HY-K1021
Manual SDS

MCE Tris-Glycine Powder (1 L of 1×) consists of Tris and Glycine, and is widely used as an electrophoresis buffer for Native-PAGE.

Tris-Glycine Powder
Size Price Stock Quantity
Free Sample (2 pouches)   Apply Now  
10 pouches USD 35 In-stock
50 pouches USD 100 In-stock

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

& Advantages

Tris-Glycine (TG) is widely used as an electrophoresis buffer for Native-PAGE. TG buffer is also used for Western Blot when prepared in 20% methanol.

MCE Tris-Glycine Powder (1 L of 1×) consists of Tris and Glycine. Each pouch can be diluted to 1 L 1× TG buffer, in which the concentration of Tris is 25 mM and the concentration of Glycine is 192 mM. This product provides a convenient way to make TG buffer and eliminates the need to weigh and mix individual components.


Store at room temperature for 3 years.


1.   Add 1 pouch of Tris-Glycine Powder into the cleaned beaker, dissolve with 600 mL distilled water (or 200 mL methanol and 400 mL distilled water when used for membrane transfer in Western Blot) under a magnetic stirrer.

2.    Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TG buffer.

Note: The pH of the 1× TG buffer is 8.3 ± 0.2 @ 25°C.

Components HY-K1021-10 pouches HY-K1021-50 pouches
Tris-Glycine Powder (1 L of 1×) 10 pouches 5 × 10 pouches
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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