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  2. Protein Biology
  3. Protein Electrophoresis & WB
  4. Electrophoresis Buffer
  5. Tris-MES-SDS Powder (1 L of 1×)

Tris-MES-SDS Powder (1 L of 1×) 

Cat. No.: HY-K1018
Manual SDS

MCE Tris-MES-SDS Powder (1 L of 1×) consists of Tris, MES, EDTA and SDS, and is mainly used for separation of small- to medium-sized proteins.

Tris-MES-SDS Powder (1 L of 1×)
Size Price Stock Quantity
Free Sample (2 pouches)   Apply Now  
10 pouches USD 90 In-stock
50 pouches USD 270 In-stock

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

& Advantages

Tris-MES-SDS (MES) is widely used in biological experiments, especially in SDS-PAGE protein electrophoresis under Bis-Tris system. Small- to medium-sized proteins can be separated effectively in this buffer.

MCE Tris-MES-SDS Powder (1 L of 1×) consists of Tris, MES, EDTA and SDS. Each pouch can be diluted to 1 L 1× MES buffer, in which the concentration of Tris is 50 mM, the concentration of MES is 50 mM, the concentration of EDTA is 1 mM and the concentration of SDS is 0.1%. This product provides a convenient way to make MES buffer and eliminates the need to weigh and mix individual components.


Store at room temperature for 3 years.


1.   Add 1 pouch of Tris-MES-SDS Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2.   Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× MES buffer.

Note: The pH of the 1× MES buffer is 7.0 ± 0.2 @ 25°C.

Components HY-K1018-10 pouches HY-K1018-50 pouches
Tris-MES-SDS Powder (1 L of 1×) 10 pouches 5 × 10 pouches
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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