Tris-MES-SDS Powder (1 L of 1×) 

Tris-MES-SDS (MES) is widely used in biological experiments, especially in SDS-PAGE protein electrophoresis under Bis-Tris system. Small- to medium-sized proteins can be separated effectively in this buffer.

MCE Tris-MES-SDS Powder (1 L of 1×) consists of Tris, MES, EDTA and SDS. Each pouch can be diluted to 1 L 1× MES buffer, in which the concentration of Tris is 50 mM, the concentration of MES is 50 mM, the concentration of EDTA is 1 mM and the concentration of SDS is 0.1%. This product provides a convenient way to make MES buffer and eliminates the need to weigh and mix individual components.

Store at room temperature for 3 years.

1.   Add 1 pouch of Tris-MES-SDS Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2.   Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× MES buffer.

Note: The pH of the 1× MES buffer is 7.0 ± 0.2 @ 25°C.