Polymyxin B (1,000×), Sterile
Based on 1 Customer Validation
MCE Polymyxin B (1,000×), Sterile (50 mg/mL) is a filtered and sterilized antibiotic solution that can be used directly in cell culture. Polymixin B is a mixture of B1 and B2 polypeptides obtained from different strains of Bacillus polymyxa, with antibacterial activity against gram-negative bacteria. It can bind lipopolysaccharide (LPS) of the outer membrane of gram-negative bacteria and disrupts the cytoplasmic membrane by inducing large pores to allow nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.
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Storage :
-20℃, 1 year. Keep away from light.
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Description & Advantages
Polymixin B is can bind lipopolysaccharide (LPS) of the outer membrane of gram-negative bacteria and disrupts the cytoplasmic membrane by inducing large pores to allow nucleotide leakage in bacterial walls. This disrupts the permeability of the cytoplasmic membrane.
Protocol
Polymyxin B (1,000×), Sterile can be used according to either of the following methods:
1. Add Polymyxin B (1,000×), Sterile to a sterile cell solution at 1 : 1,000 (v/v) and mix thoroughly before use.
2. Add Polymyxin B (1,000×), Sterile to cell culture medium at 1 : 1,000 (v/v), mix thoroughly, and filter-sterilize.
Note: The product contains 50 mg/mL Polymyxin B sulfate in ddH2O. A common working concentration is 50 mg/L. Avoid repeated freeze-thaw cycles and use sterile reagents, tubes, and tips.
Storage
-20℃, 1 year.
Keep away from light.
Components
| Components | HY-K1044-10 mL | HY-K1044-100 mL |
|---|---|---|
| Polymyxin B (1,000×), Sterile | 10 mL | 100 mL |
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