1. Cell Cycle/DNA Damage
    Anti-infection
  2. Antifolate
    Bacterial
  3. Trimethoprim

Trimethoprim 

Cat. No.: HY-B0510 Purity: 99.98%
Handling Instructions

Trimethoprim is a bacteriostatic antibiotic used mainly in the prophylaxis and treatment of urinary tract infections.

For research use only. We do not sell to patients.

Trimethoprim Chemical Structure

Trimethoprim Chemical Structure

CAS No. : 738-70-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
500 mg USD 50 In-stock
Estimated Time of Arrival: December 31
5 g USD 60 In-stock
Estimated Time of Arrival: December 31
10 g USD 72 In-stock
Estimated Time of Arrival: December 31
50 g   Get quote  

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Customer Review

Based on 2 publication(s) in Google Scholar

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Description

Trimethoprim is a bacteriostatic antibiotic used mainly in the prophylaxis and treatment of urinary tract infections. Target: DHFR Trimethoprim (TMP), an inhibitor of dihydrofolate reductase, decreases the level of tetrahydrofolate supplying one-carbon units for biosynthesis of nucleotides, proteins, and panthotenate. TMP caused induction of DnaK, DnaJ, GroEL, ClpB, and IbpA/B Hsps. Among these Hsps, IbpA/B were most efficiently induced by TMP and coaggregated with the insoluble proteins [1]. Trimethoprim binds to dihydrofolate reductase and inhibits the reduction of dihydrofolic acid (DHF) to tetrahydrofolic acid (THF). THF is an essential precursor in the thymidine synthesis pathway and interference with this pathway inhibits bacterial DNA synthesis. Trimethoprim's affinity for bacterial dihydrofolate reductase is several thousand times greater than its affinity for human dihydrofolate reductase. Sulfamethoxazole inhibits dihydropteroate synthetase, an enzyme involved further upstream in the same pathway. Trimethoprim and sulfamethoxazole are commonly used in combination due to their synergistic effects. This drug combination also reduces the development of resistance that is seen when either drug is used alone [2].

Clinical Trial
Molecular Weight

290.32

Formula

C₁₄H₁₈N₄O₃

CAS No.

738-70-5

SMILES

NC1=NC=C(CC2=CC(OC)=C(OC)C(OC)=C2)C(N)=N1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (172.22 mM; Need ultrasonic)

H2O : 0.67 mg/mL (2.31 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4445 mL 17.2224 mL 34.4447 mL
5 mM 0.6889 mL 3.4445 mL 6.8889 mL
10 mM 0.3444 mL 1.7222 mL 3.4445 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.61 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.61 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.61 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Trimethoprim
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HY-B0510
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