Mouse Monoclonal Antibody Custom Services

  • High Success Rate
  • Flexible Customization
  • Strict Quality Control

Customization Process

MCE provides high-quality mouse monoclonal antibody customization services covering the full workflow: antigen design, animal immunization, antibody purification, and titer detection to support the success of your research projects.

  • Project Consultation
  • Protocol Confirmation
  • Antigen Design and Preparation
  • Animal Immunization
  • Cell Fusion
  • Positive Clone Screening
  • Antibody Purification and Production
  • Delivery

Technical Route

[1] Mol Biomed. 2024 Oct 11;5(1):47. doi: 10.1186/s43556-024-00210-1.

Technical Advantages

MCE adopts industry-leading technical platforms and quality standards to ensure excellent antibody performance.

  • High Success Rate

    With extensive experience in antigen design, the immunization success rate exceeds 90%, ensuring smooth project progression.

  • Flexible Customization

    Supports customized immunization protocols, purification methods, and detection requirements tailored to your experimental needs.

  • Strict Quality Control

    Each project undergoes strict quality control with a complete Certificate of Analysis (COA).

Service Commitment

  • Professional technical consultation and protocol design
  • Transparent pricing and clear timelines
  • Strict quality control and complete reports
  • Comprehensive after-sales support and technical guidance

Service Content and Description

Service Item Product Name Deliverables Lead Time
Antigen Preparation Synthetic Peptide: Chemical peptide synthesis based on selected sequences; carrier proteins such as KLH and BSA can be conjugated to enhance immunogenicity. 1.Purified antibody: 1–2 clones, 5–10 mg per clone;
2.Remaining antigen;
3.Certificate of Analysis (COA). 		2–3 weeks
Recombinant Protein Expression: Target protein or fragment expression using prokaryotic (e.g., Escherichia coli) or eukaryotic (e.g., mammalian cells) systems, followed by purification and identification. 4–5 weeks
Animal Immunization 1.Mice are immunized via multiple subcutaneous injections, 3–5 times in total.
2.Post-immunization blood collection from the retro-orbital venous plexus; serum antibody titer determined by indirect ELISA.		 6–9 weeks
Hybridoma Cell Line Establishment 1.Myeloma cell resuscitation;
2.Immunized spleen cell preparation;
3.Cell fusion and plating;
4.Positive hybridoma cell screening;
5.Positive hybridoma cell cloning;
6.Positive monoclonal cell line establishment and preservation.		 4–8 weeks
Antibody Purification Ascites production, purification, and quality control 3–4 weeks

Customer Requirements

1.Completed antibody customization form including species, sequence, and application of the target gene.

2.Antigen provided by customer: (optional)

Protein: purity >85%, concentration >1 mg/mL, 3–5 mg purified protein.

Successful Cases

MCE has provided high-quality antibody customization services for hundreds of research institutions and biopharmaceutical companies.

*All case demonstrations have been approved by customers.

Customization Form

Each antibody customization project will be evaluated to determine the corresponding protocol and price. For more information on service prices or technical details, please email us at [email protected] or contact MCE sales representatives directly.

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