1. MCE Kits
  2. Annexin V-PE Apoptosis Detection Kit

Annexin V-PE Apoptosis Detection Kit 

Cat. No.: HY-K1075
SDS

MCE Annexin V-PE Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

Annexin V-PE Apoptosis Detection Kit
Size Price Stock
20 T USD 290 Ask For Quote & Lead Time
50 T USD 494 Ask For Quote & Lead Time

* Please select Quantity before adding items.

  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE Annexin V-PE Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. In normal live cells, Phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. Upon initiation of apoptosis, PS is translocated from the inner to the outer leaflet of the membrane. Annexin V is a 35-36 kDa Ca2+ -dependent phospholipid-binding protein that has a high affinity for PS. After staining cells with Annexin V-PE, live cells show little or no fluorescence, apoptosis cells and necrosis cells show red fluorescence.

Storage

-20℃, 1 year

Protect from light

Avoid repetitive freeze-thaw cycles

Protocol

1. Collect 1-5 × 105 cells

For suspension cells: Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

For adherent cells: Collect the cell culture medium. Wash cells with PBS and add trypsin to dissociate cells. Add the medium and gently suspend the cells to make a single-cell suspension. Centrifuge at 1000 g for 5 minutes and then discard the supernatant. Add 1 mL of pre-cooled PBS to resuspend the cells, centrifuge at 1000 g for 5 minutes and then discard the supernatant.

Note: It is recommended to use trypsin containing no EDTA.

2. Resuspend the cells in 195 μL of Binding Buffer.

3. Add 5 μL of Annexin V-PE.

4. Incubate the cells at room temperature for 10-20 minutes in the dark.

5. Detect the fluorescence by flow cytometer or fluorescence microscope (Ex = 488 nm; Em = 578 nm).

Components
Components HY-K1075-20T HY-K1075-50T
Annexin V-PE 100 μL 250 μL
Binding Buffer 12 mL 30 mL
Documentation

Your Recently Viewed Products:

Bulk Inquiry

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Annexin V-PE Apoptosis Detection Kit
Cat. No.:
HY-K1075
Quantity:
MCE Japan Authorized Agent: