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Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)

258 Cited Publications
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Based on 258 publication(s) in Google Scholar

MCE Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) is a blend of 5 pan-protease inhibitors for protection of protein integrity. The 25 pcs is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :

    4℃, 2 years

  • Experimental Validation Results for Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)
  • Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) Product Image
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Description & Advantages

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.

MCE Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) is a blend of 5 pan-protease inhibitors for protection of protein integrity. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.

Protocol

1. Direct Use: Add one tablet of the Protease Inhibitor Cocktail to 10 mL of the sample and mix thoroughly until dissolved.

2. Preparation of Stock Solution: Dissolve one tablet of the Protease Inhibitor Cocktail in 1 mL of ddH2O, vortex to ensure complete dissolution, and prepare a 10× stock solution. Store at 4°C for up to one week or at -20°C for at least one month.

Storage

4℃, 2 years

Components

Cat. No. Product Name Package
HY-K0011-25 pcs Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) 25 pcs
HY-K0011-50 pcs Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) 25 pcs × 2

Documentation

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Minimum order quantity
100 mg

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  • Experimental Validation Results for Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)

    Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) purchased from MedChemExpress. Usage Cited in: Mol Cell. 2024 Jun 20;84(12):2304-2319.e8. [Abstract]

    Expression of PABPC1 in H9 cells and FB neurons (A), H9 cells, and SH-SY5Y cells (B), revealed by western blotting. ACTIN was detected as control.

Assay Types
  • Experimental Validation Results for Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)
    1/16

    Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) purchased from MedChemExpress. Usage Cited in: Mol Cell. 2024 Jun 20;84(12):2304-2319.e8. [Abstract]

    Expression of PABPC1 in H9 cells and FB neurons (A), H9 cells, and SH-SY5Y cells (B), revealed by western blotting. ACTIN was detected as control.

  • Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) Product Image
    1/16
  • Experimental Validation Results for Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)
  • Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) Product Image