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  2. Protease Inhibitor Cocktail, mini-Tablet

Protease Inhibitor Cocktail, mini-Tablet  (EDTA-Free)

Cat. No.: HY-K0011
Download Inhibitor Cocktail Flyer Manual COA SDS

Protease inhibitor cocktail is used in cell lysates or tissue extracts to increase protein stability.

Protease Inhibitor Cocktail, mini-Tablet
Size (mini-Tablet) Price Stock Quantity
Free Sample   Apply now  
25 pcs USD 120 In-stock
Estimated Time of Arrival: December 31
50 pcs USD 200 In-stock
Estimated Time of Arrival: December 31

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Top Publications Citing Use of Products

Customer Validation

  • Protein: Bcl-2

    Cell Line: thyroid cancer cell line 8505C

    MCE Protease Inhibitor Cocktail provides Bcl-2 protection in thyroid cancer cell line 8505C, and is most effective after 6-12 hours incubation at room temperature.

  • Protein: ERK1/2

    Cell Line: K1 cell line

    MCE Protease Inhibitor Cocktail (mini-Tablet) protects ERK1/2 in K1 cell line, and is most effective after 6-12 hours incubation at room temperature.

MCE Protease Inhibitor Cocktail provides Bcl-2 protection in thyroid cancer cell line 8505C, and is most effective after 6-12 hours incubation at room temperature.

 

MCE Protease Inhibitor Cocktail (mini-Tablet) protects ERK1/2 in K1 cell line, and is most effective after 6-12 hours incubation at room temperature.

 
  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases. Generally, protease inhibitor cocktails are used in cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. MedChemExpress Protease Inhibitor Cocktail (EDTA free, mini-Tablet) is a blend of 5 pan-protease inhibitors for protection of protein integrity.

Storage
Protease Inhibitor Cocktail (EDTA free, mini-Tablet) can be stored at 4°C for 2 years.
Protocol

1. This product can be applied in Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
2. One mini-Tablet can be added to 10mL extraction medium directly. Alternatively, a stock solution (10X conc. one mini-Tablet in 1mL ddH2O) can be prepared. Vortex briefly to accelerate the dissolution. The stock solution is stable for one week when stored at 4°C, and for at least 4 weeks at -20°C.

Components
Ingredient Target Inhibitor Type
AEBSF Serine proteases Irreversible
Aprotinin Serine proteases Reversible
Bestatin Aminopeptidases Reversible
E-64 Cysteine proteases Irreversible
Leupeptin Serine and cysteine proteases Reversible
Documentation

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Product Name:
Protease Inhibitor Cocktail, mini-Tablet
Cat. No.:
HY-K0011
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