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IRE1 alpha Antibody (YA6022)

(Synonyms: ERN1; IRE1; Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a)
Customer Review

Based on 1 Customer Validation

IRE1 alpha Antibody (YA6022) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRE1 alpha.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • IRE1 alpha Antibody (YA6022) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:100-1:500 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

IRE1 alpha Antibody (YA6022) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRE1 alpha.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 110 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

RRID

AB_3719120

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Western blot analysis of extracts from HEK293 (lane2(20μg), Neuro-2a (lane3(20μg), C6 (lane4(20μg) and Hela (lane5(20μg) using IRE1 alpha Antibody (HY-P86330). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

  • Function

    IRE1 alpha is a Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A[1][2][3][4][5][6][7][8][9][10][11][12].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Widely expressed. High expression levels are observed in pancreatic tissue.

    Induction:Induced by the ER stressor tunicamycin

  • Isoforms & Post-Translational Modification

    O75460 has 2 isomers: O75460-1: 109735 Da (predicted); O75460-2: 6649 Da (predicted).
    Autophosphorylated following homodimerization. Autophosphorylation promotes activation of the endoribonuclease domain (PubMed:12637535, PubMed:21317875, PubMed:28128204, PubMed:9637683). In response to ER stress, phosphorylated at Ser-724, Ser-729 and possibly Ser-726; phosphorylation promotes oligomerization and endoribonuclease activity (PubMed:30118681). Dephosphorylated at Ser-724, Ser-729 and possibly Ser-726 by RPAP2 to abort failed ER-stress adaptation and trigger apoptosis (PubMed:30118681). Phosphorylated at Ser-724; in response to the ER stressor tunicamycin (PubMed:36739529);ADP-ribosylated by PARP16 upon ER stress, which increases both kinase and endonuclease activities

  • Subunit

    Monomer (PubMed:16973740, PubMed:29198525). Homodimer; disulfide-linked; homodimerization takes place in response to endoplasmic reticulum stress and promotes activation of the kinase and endoribonuclease activities (PubMed:12637535, PubMed:16973740, PubMed:21317875, PubMed:24508390, PubMed:30118681). Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges (PubMed:12637535). Interacts (via the luminal region) with DNAJB9/ERdj4; interaction takes place in unstressed cells and promotes recruitment of HSPA5/BiP (PubMed:29198525). Interacts (via the luminal region) with HSPA5/BiP; HSPA5/BiP is a negative regulator of the unfolded protein response (UPR) that prevents homodimerization of ERN1/IRE1 and subsequent activation of the protein (PubMed:12637535, PubMed:29198525, PubMed:36739529). Interaction with HSPA5 also competitively inhibits ERN1 interaction with MANF (PubMed:36739529). Interacts with PDIA6, a negative regulator of the UPR; the interaction is direct and disrupts homodimerization (PubMed:24508390). Interacts with DAB2IP (via PH domain); the interaction occurs in a endoplasmic reticulum stress-induced dependent manner and is required for subsequent recruitment of TRAF2 to ERN1/IRE1 (By similarity). Interacts with TAOK3 and TRAF2 (PubMed:11278723). Interacts with RNF13 (PubMed:23378536). Interacts with LACC1 (PubMed:31875558). Interacts (when unphosphorylated) with DDRGK1; interaction is dependent on UFM1 and takes place in response to endoplasmic reticulum stress, regulating ERN1/IRE1-alpha stability (PubMed:28128204). Interacts (via N-terminus) with P4HB/PDIA1; the interaction is enhanced by phosphorylation of P4HB by FAM20C in response to endoplasmic reticulum stress and results in attenuation of ERN1 activity (PubMed:32149426). Interacts with TMBIM6; this interaction inhibits ERN1 activity (PubMed:19328063). Interacts (via luminal domain) with MANF (via C-terminus); the interaction is decreased in the presence of increasing concentrations of Ca(2+) (PubMed:36739529)

  • SwissProt ID

    O75460

  • Gene ID
    2081 [NCBI]
  • Synonyms

    ERN1; IRE1; Serine/threonine-protein kinase/endoribonuclease IRE1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a

IRE1 alpha Antibody (YA6022) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane2(20μg), Neuro-2a (lane3(20μg), C6 (lane4(20μg) and Hela (lane5(20μg) using IRE1 alpha Antibody (HY-P86330). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image
    1/16
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • IRE1 alpha Antibody (YA6022) Product Image
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane2(20μg), Neuro-2a (lane3(20μg), C6 (lane4(20μg) and Hela (lane5(20μg) using IRE1 alpha Antibody (HY-P86330). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • Experimental Validation Results for IRE1 alpha Antibody (YA6022)
  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane2(20μg), Neuro-2a (lane3(20μg), C6 (lane4(20μg) and Hela (lane5(20μg) using IRE1 alpha Antibody (HY-P86330). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using IRE1 alpha Antibody (YA6022). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86330, 1/50) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • IRE1 alpha Antibody (YA6022) Product Image WB
  • IRE1 alpha Antibody (YA6022) Product Image IHC-P
  • IRE1 alpha Antibody (YA6022) Product Image IHC-P
  • IRE1 alpha Antibody (YA6022) Product Image IHC-P
  • IRE1 alpha Antibody (YA6022) Product Image IHC-P
  • IRE1 alpha Antibody (YA6022) Product Image IHC-P
  • IRE1 alpha Antibody (YA6022) Product Image IHC-P
  • IRE1 alpha Antibody (YA6022) Product Image IHC-P