Human iPSC/ESC Cortical Brain Organoid Induction Differentiation Kit 

MCE Human iPSC/ESC Cortical Brain Organoid Induction Differentiation Kit is a standardized culture system specifically designed to recapitulate the developmental processes and functional features of the human cerebral cortex through forebrain ventralization-based signaling regulation. By sequentially activating the Wnt/β-catenin pathway and gradually inhibiting BMP/Smad signaling, this system efficiently drives human pluripotent stem cells (PSC) to differentiate into high-purity glutamatergic neurons (VGLUT1/2⁺ > 85%), while simultaneously promoting the formation of Pax6⁺/BLBP⁺ radial glial cells that establish a biomimetic ventricular-zone–like structure.

Within 60 d, the medium supports the robust generation of layered cortical organoids measuring 1.5-2.0 mm in diameter. These organoids exhibit characteristic cortical lamination, including deep-layer TBR1⁺ (layers V-VI) and upper-layer SATB2⁺ (layers II-IV) neurons. In addition, the system induces MBP⁺/MOG⁺ oligodendrocytes that form dense myelinated structures, with LFB staining positivity exceeding 70%.

This organoid model is well suited for a wide range of neurological studies, including Alzheimer’s disease and tauopathy modeling, NMDA receptor dysfunction in schizophrenia, and synaptic plasticity alterations associated with autism spectrum disorders. It is also compatible with single-cell sequencing and calcium-imaging–based functional profiling, providing a robust and efficient platform for investigating neural development and disease mechanisms.

-20°C, 2 years.

1. This product is sterile and should be handled using aseptic techniques. It is recommended to aliquot and store to avoid contamination.

2. This product is for R&D use only, not for drug, household, or other uses.

3. For your safety and health, please wear a lab coat and disposable gloves to operate.