Tissue Dissociation Enzyme

Collagenase, Type IV (EC 3.4.24.3) is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type IV degrades type IV collagen and type VII collagen, the main components of the basement membrane, and can also decompose basement matrix and elastin.

Collagenase, Type II is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type II breaksdown collagens1, 3, 5, 7, 8, 10, fibronectin, gelatin, aggrecann.

Collagenase, Type I is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type I breaks down collagens 1, 3, 7, 8, 10, gelatin, proteoglycans, aggrecan.

Neutral protease, Paenibacillus polymyxa (Dispase II, Dispase) is a neutral protease and potent fibronectinase and type IV collagenase. Neutral protease, Paenibacillus polymyxa can be used to separate the intact epidermis from the dermis and intact epithelial sheets in culture from the substratum.

Collagenases are enzymes that break the peptide bonds in collagen. Collagenases are derived from the Clostridium histolyticum. Collagenases (Type I) are proteolytic enzymes that break peptide bonds in collagen and can be used for tissue digestion and dissociation.

Thermolysin (Geobacillus stearothermophilus (EC 3.4.24.27)) is a thermostable neutral metalloproteinase. It requires one zinc ion to maintain enzyme activity and four calcium ions to maintain structural stability. Thermolysin (Geobacillus stearothermophilus (EC 3.4.24.27) specifically catalyzes the hydrolysis of peptide bonds containing hydrophobic amino acids.

Lysozyme Biotin-Caproyl against immobilized avidin >95%. Contains approx. two moles biotin to one mole of lysozyme.

Collagenase, Type VIII is a mixed enzyme derived from Clostridium histolyticum that contains collagenase, a nonspecific protease, and a clostripain. Collagenase, Type VIII can hydrolyze Type VIII collagen and may be used to study the formation of atherosclerosis. Type VIII collagen is a regulator of endothelial cell differentiation and angiogenesis, a substrate for cell adhesion and migration such as smooth muscle cells, and may accumulate in atherosclerosis. After endotoxin activates the expression of Collagenase, Type VIII, it can reduce the production of Type VIII collagen and has the potential to inhibit atherosclerosis.

Collagenase, Type III is a microbially derived matrix metalloproteinases (MMPs) and zinc peptidase. Collagenase, Type III breaksdown collagens1, 4, 9, 10, 14, fibronectin, MMP-9, gelatin, plasminogen, aggrecan,perlecan osteonectin

Zymolyase, Arthrobacter luteus, is a zymolyase mainly found in Arthrobacter luteus. Enzyme, an enzyme with beta-1,3 glucanase activity, removes the electron-dense outer layer of the Plasmodium karinii cell wall, exposing an electron-lucent layer.

Pectinase (EC 3.2.1.15) is a mixed enzymes that hydrolyze pectic substances, it mostly presents in microorganisms and higher plants. Pectinase is involved in the metabolism of the cell wall as well as in the growth of the cell, senescence, ripening of fruits, pathogenesis and abscission process.

Collagenase, Type VII (EC 3.4.24.3) is a collagenase that can be used to induce thalamic hemorrhage.

Snailase, Snail gastrointestinal is an enzyme mixture composed of more than 20 enzymes, which is often used for enzymatic hydrolysis of purified flavonoid glycosides. Snailase can be obtained from the digestive tract and includes cellulase, sucrase, hemicellulase, pectinase, polygalacturonase, protease, etc.

Collagenase (Type D, animal free) does not contain animal-related components, and has collagenase and secondary protease activities similar to type 1 and type 2 collagenase. The activity of Collagenase (Type D, animal free) is higher than that of Collagenase (Type A, animal free).

Collagenase, Type VI (EC 3.4.24.3) is a collagenase that can degrade type VI collagen. Type VI collagen is a component of cell membranes in various tissues (such as skin, heart, blood vessels, cartilage, and synovial fluid). Excessive collagenase can cause extracellular matrix lesions. Collagenase is also a biomarker for tumor invasion and metastasis. Collagenase, Type VI can specifically act on the peptide bond between proline and glycine. This feature can be used to quickly and sensitively detect its concentration level in experiments using corresponding modified electrodes.

Hyaluronidase (human recombinant) (Vorhyaluronidase alfa; rHuPH 20) is an enzyme that catalyzes the degradation of hyaluronic acid. Hyaluronidase (human recombinant) is used to improve the absorption and dispersal of parenteral fluids, medications, and contrast media.

Hyaluronidase, Ovine testes is an endoglycosidase. Hyaluronidase, Ovine testes specifically degrades Hyaluronic acid (HY-B0633A) and Chondroitin sulfate (HY-B2162) by hydrolyzing β-glycosidic bonds in acidic mucopolysaccharides. Hyaluronidase, Ovine testes disperses follicular cells during fertilization by breaking down the hyaluronic acid-rich cumulus. Hyaluronidase, Ovine testes can be used in the study of fertility-related diseases.

Cellulase R-10 from Trichoderma Vride is an enzyme mixture that includes cellulase, hemicellulase, pectinase and protease, etc. Cellulase R-10 from Trichoderma Vride can act on the 1,4-β-D-glucosidic bonds in cellulose, catalyzing the hydrolysis of cellulose molecules and generating cellulose oligosaccharides, cellulose di-saccharides and glucose. Cellulase R-10 from Trichoderma Vride can be used in combination with lyase R-10 to break down the cell walls of plants, preparing protoplasts. Cellulase R-10 from Trichoderma Vride is widely applied in plant cell hybridization research.

Lysozyme (Muramidase) is a conserved antimicrobial protein. Lysozyme exerts its bactericidal effect by hydrolyzing bacterial cell wall peptidoglycan (PG). Lysozyme plays an important role in limiting bacterial growth on mucosal surfaces and other sites, not only controlling potential pathogens but also limiting overgrowth of microbiota to prevent dysbiosis. Extracellular lysozyme can also degrade polymeric PG into soluble fragments, activate NOD receptors in mucosal epithelial cells, and lead to the secretion of chemokines and activating factors by neutrophils and macrophages.

Driselase, Basidiomycetes sp, a complex mixt. of wall-digesting enzymes, is a specific commercial fungal protoplasting enzyme preparation. Driselase can be used in combination with lyase to promote protoplast formation in fungi. Driselase is by far the most potent of the enzymes tested for polysaccharide digestion and greatly increases both tensile and indentation compliances, yet it does not induce wall creep, even after 6 h of digestion.