Neutral protease, Paenibacillus polymyxa
Based on 7 publication(s) in Google Scholar
Neutral protease, Paenibacillus polymyxa (Dispase II, Dispase) is a neutral protease and potent fibronectinase and type IV collagenase. Neutral protease, Paenibacillus polymyxa can be used to separate the intact epidermis from the dermis and intact epithelial sheets in culture from the substratum.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- CAS 番号: 42613-33-2
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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生物活性
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Technical Parameters
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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MedChemExpress（MCE）の使用を引用している文献 Neutral protease, Paenibacillus polymyxa
MoreEndogenous Metabolite アイソフォーム固有の製品をすべて表示
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生物活性
製品説明
体外実験
Protocol
1. Reconstitute Dispase
1) Dissolve the non-sterile enzyme in ddH2O to 10 mg/mL. Filter sterilize through a 0.22 μm filter membrane.
2) Further dilute with DPBS without calcium and magnesium to a final concentration of 0.6-2.4 U/mL.
Note: Concentrations higher than 2.4 U/mL are not recommended.
2. Dissociate Tissue
1) Mince tissue into 3-4 mm pieces with a sterile scalpel or scissors.
2) Wash the tissue pieces several times in sterile PBS without calcium and magnesium.
3) Submerge tissue fragments in Dispase solution (0.6-2.4 U/mL) and incubate at 37°C.
4) Stir slowly at 37°C until the tissue is sufficiently dissolved.
Note: For compact tissues, we recommend incubating for 1 hour. Cells will not be adversely affected even after several hours in Dispase.
5) If necessary, separate the dispersed cells from residual tissue by passing the mixture through a sterile stainless steel or nylon mesh or simply decant the cells after larger fragments have settled. Fresh Dispase solution may be added to the remaining tissue fragments if further disaggregation is required.
6) Pellet cells by centrifugation and decant the enzyme solution.
7) Seed cells into culture vessels containing appropriate culture medium and incubate under predetermined conditions.
3. Subculture Cells
1) Aspirate culture medium and cover the cells with Dispase solution, prewarmed to 37°C. Incubate for 5 minutes at 37°C.
2) Decant the Dispase solution and incubate the cells for an additional 10 minutes at 37°C.
3) Monitor cell detachment using an inverted microscope. If necessary, incubate for an additional 15 minutes or until detachment is complete.
4) Suspend the cells in culture medium and pellet by centrifugation.
5) Resuspend the cells in a fresh culture medium. Plate the cells as usual.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Enzyme Activity
≥ 0.5 U/mg soild
Unit Definition
One unit is defined as the amount of enzyme that hydrolyze casein to produce color equivalent to 1.0 μmole(181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Technical Parameters
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Biological Source
Paenibacillus polymyxa
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Activators
Ca²⁺, Mg²⁺, Mn²⁺ and divalent cations including Fe²⁺
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Inhibitors
EDTA, EGTA, Hg²⁺ and other heavy metals
化学情報
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CAS 番号 42613-33-2
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性状 Solid
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Color White to off-white
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SMILES
[Neutral protease, Paenibacillus polymyxa]
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別名
Dispase II, Dispase
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Structure Classification
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Initial Source
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (7)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
The role of vitamin K and its antagonist in the process of ferroptosis-damaged RPE-mediated CNV. [Abstract]2025 Mar 20;16(1):190. PMID: 40108164 -
Cell Signal
The role of TRAF6 in regulating MAP3K7/P38/MAPK pathway in the epithelial-mesenchymal transition of retinal pigment epithelial cells in proliferative vitreoretinopathy. [Abstract]2026 Oct:146:112670. PMID: 42285191 -
J Dig Dis
Intestinal NLRP3 Deficiency Exacerbates MASLD in Male Mice via Reduced Butyrate Production. [Abstract]2025 Dec 16. PMID: 41400298 -
Gene
Static magnetic field contributes to osteogenic differentiation of hPDLSCs through the H19/Wnt/β-catenin axis. [Abstract]2025 Jan 15:933:148967. PMID: 39341520 -
溶剤 & 溶解度
体外:
H2O : ≥ 50 mg/mL
* "≥" means soluble, but saturation unknown.
純度とドキュメンテーション
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データシート (269 KB)
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SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Stenn KS, et al. Dispase, a neutral protease from Bacillus polymyxa, is a powerful fibronectinase and type IV collagenase. J Invest Dermatol. 1989 Aug;93(2):287-90. [Content Brief]
[2]. Calvo B, et al. Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometry. IBRO Rep. 2020 Jan 13;8:36-47. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Neutral protease, Paenibacillus polymyxa 関連分類
HY-131577 関連分類
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