Mito Red Kit
Based on 1 publication(s) in Google Scholar
Mito Red is a red-fluorescent fluorescent probe that specifically labels mitochondria in living mammalian cells.
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Storage :
-20°C, 1 year
Keep away from light
Description & Advantages
Mito Red is a red-fluorescent fluorescent probe that specifically labels mitochondria in living mammalian cells and is highly water-soluble and pH-stable; it has a maximum excitation wavelength of 550 nm and a maximum emission wavelength of 575 nm. The MCE Mito Red kit enables the specific labeling of mitochondria in living cells, with stable fluorescence, and is compatible with labeling in fixed cells.
Protocol
1. Prepare the cells.
2. Prepare the working solution for incubation.
3. Wash the cells growing in the well plate or confocal dish with appropriate amount of PBS, and then take appropriate amount of serum-free medium to rinse the cell surface once.
4. Add the working solution and incubate the cells with the working solution for 1 h.
5. Take the appropriate amount of PBS and wash 3 times, add the appropriate amount of serum-free medium and leave for 15 min.
6. Take appropriate amount of PBS and wash 3 times, replace with serum cell culture medium and leave for 15 min.
7. Imaging.
Publications
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Journal Impact Factor
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Most Recent
Storage
-20°C, 1 year
Keep away from light