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Cell Cycle and Apoptosis Analysis Kit (PI staining)

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Based on 30 publication(s) in Google Scholar

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.


  • Storage :

    4℃, 6 months; -20℃,1 year.


    Protect from light.


  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Cell Cycle and Apoptosis Analysis Kit (PI staining) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Description & Advantages

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis. The content of DNA is changed with the process of cell cycle. DNA can be stained by fluorescent dye, such as Propidium Iodide (PI), to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1/S/G2/M phase as well as apoptosis cells with signals at sub-G1 region. PI is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. However, PI also binds to RNA, RNase A provided in this kit can eliminate RNA interference. In addition, light scattering also changes during apoptosis. In early apoptosis, light signals from Forward Scatter (FSC) become weak and signals from Side Scatter (SSC) increase or remain unchanged. When cells proceed to the phase of late apoptosis, signals from FSC/SSC both decrease.


Protocol

1. Collect approximately 1 × 106 cells, wash with PBS, and centrifuge to obtain the cell pellet.

2. While gently vortexing the cells, slowly add pre-cooled 70% ethanol to disperse and fix the cells. Fix at 4°C for at least 2 h or overnight.

3. Centrifuge to remove ethanol and wash cells with PBS to remove residual ethanol.

4. Prepare PI/RNase A staining solution as follows:

Component Per sample
Cell Stain Buffer 500 μL
PI Stain (20×) 25 μL
RNase A (50×) 10 μL

5. Thoroughly resuspend cells in the staining solution and incubate at 37°C for 30 min protected from light.

6. Detect PI fluorescence by flow cytometry. Acquire at least 10,000 valid cell events per sample when possible. Analyze DNA content to determine G0/G1, S, G2/M distributions and sub-G1 apoptotic cells.

Storage

4℃, 6 months; -20℃,1 year.


Protect from light.


Components

Components HY-K1071-50T
Cell Stain Buffer 25 mL
PI Stain (20×) 1.25 mL
RNase A (50×) 0.5 mL

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100 mg

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  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Experimental Validation Results for Cell Cycle and Apoptosis Analysis Kit (PI staining)
  • Cell Cycle and Apoptosis Analysis Kit (PI staining) Product Image