2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Apoptosis and Necrosis
  6. Cell Cycle and Apoptosis Analysis Kit (PI staining)

Cell Cycle and Apoptosis Analysis Kit (PI staining) 

Cat. No.: HY-K1071
Manual COA SDS Technical Support

MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis.


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    Cell Cycle and Apoptosis Analysis Kit (PI staining) purchased from MCE. Usage Cited in: Cell Death Dis. 2025 Dec 23.  [Abstract]

    Representative flow cytometry plots of cell cycle distribution in WERI-Rb1 NTC cells and WERI-Rb1 sgELF2 cells were shown.

    Cell Cycle and Apoptosis Analysis Kit (PI staining) purchased from MCE. Usage Cited in: Cell Biol Toxicol. 2024 Dec 4;40(1):108.  [Abstract]

    Cell apoptotic rate was assessed by flow cytometry.

    Cell Cycle and Apoptosis Analysis Kit (PI staining) purchased from MCE. Usage Cited in: Int J Mol Sci. 2025 Apr 30;26(9):4274.

    Apoptosis assays, with sgNC and sgUso1 in GC1 and GC2 cells, were performed.

    Cell Cycle and Apoptosis Analysis Kit (PI staining) purchased from MCE. Usage Cited in: Cancer Cell Int. 2024 Oct 14;24(1):337.  [Abstract]

    PI staining of PUS7-depleted B-CPAP cells or Nthy ori 3-1 cells was performed.

    Cell Cycle and Apoptosis Analysis Kit (PI staining) purchased from MCE. Usage Cited in: Pharmaceuticals (Basel). 2024 Sep 26;17(10):1269.  [Abstract]

    Apoptosis was detected by flow cytometry using Annexin V and PI staining methods in MDA-MB-231 and MDA-MB-468 cells after treatment with NF113 or TAX (Taxol) for 48 h.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    MCE Cell Cycle and Apoptosis Analysis Kit (PI staining) provides a convenient method to detect cell cycle and cell apoptosis. The content of DNA is changed with the process of cell cycle. DNA can be stained by fluorescent dye, such as Propidium Iodide (PI), to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1/S/G2/M phase as well as apoptosis cells with signals at sub-G1 region. PI is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. However, PI also binds to RNA, RNase A provided in this kit can eliminate RNA interference. In addition, light scattering also changes during apoptosis. In early apoptosis, light signals from Forward Scatter (FSC) become weak and signals from Side Scatter (SSC) increase or remain unchanged. When cells proceed to the phase of late apoptosis, signals from FSC/SSC both decrease.


    Storage

    4℃, 6 months; -20℃,1 year.


    Protect from light.
    Components
    Components HY-K1071-50T
    Cell Stain Buffer 25 mL
    PI Stain (20×) 1.25 mL
    RNase A (50×) 0.5 mL
    Documentation

    Manual (461 KB) SDS (251 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cell apoptosis kitCell cycle kitCell apoptosis stainingCell apoptosis detectionCell cycle detection

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