One Step TUNEL Apoptosis Detection Kit (Cyanine 3)
Based on 7 publication(s) in Google Scholar
MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show red fluorescence.
-
Storage :
-20°C, 1 year. Protect from light.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180-200 bp fragments. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope or flow cytometer. The maximum excitation wavelength and emission wavelength of Cy3 (Cyanine 3) were 550 nm and 570 nm respectively.
Protocol
1. Preparation of TUNEL working solution
Prepare the TUNEL working solution on ice and protected from light immediately before use:
| Component | Per sample (50 μL) |
|---|---|
| TdT Enzyme | 5 μL |
| Cy3-dUTP Labeling Mix | 45 μL |
Note: For a negative control, omit TdT Enzyme and replace it with an equal volume of TdT Dilution Buffer.
2. Adherent cells
1. Wash cells twice with PBS for 5 min each.
2. Fix with 4% paraformaldehyde/PBS for 30 min at 4°C.
3. Wash twice with PBS for 5 min each.
4. Permeabilize with PBS containing 0.3% Triton X-100 for 5 min at room temperature.
5. Wash twice with PBS for 5 min each.
6. Add 50 μL TUNEL working solution per sample and incubate at 37°C for 60 min protected from light.
7. Wash three times with PBS and observe by fluorescence microscopy. Cy3 has maximum excitation/emission wavelengths of approximately 550/570 nm, and TUNEL-positive apoptotic cells show red fluorescence.
3. Suspension cells
1. Collect approximately 2 × 106 cells by centrifugation and wash twice with PBS for 5 min each.
2. Fix for 30 min at 4°C, gently mixing during fixation to reduce cell clumping.
3. Wash twice with PBS for 5 min each.
4. Resuspend in permeabilization solution and incubate for 5 min at room temperature.
5. Wash twice with PBS for 5 min each.
6. Add 50 μL TUNEL working solution and incubate at 37°C for 60 min protected from light.
7. Wash with PBS and resuspend for Cy3 fluorescence analysis by flow cytometry or fluorescence microscopy.
Publications
-
Journal Impact Factor
-
Most Recent
-
Adv Sci (Weinh)Faecalibaculum rodentium Alleviates Ionizing Radiation-Induced Damage in Mice by Improving Intestinal Integrity and Hematopoiesis via Its Metabolite Butyrate. [Abstract]2025 Oct 13:e09383. PMID: 41082369
One Step TUNEL Apoptosis Detection Kit (Cyanine 3) purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Oct 13:e09383. [Abstract]
The numbers of apoptotic c‐Kit+ cells isolated from either control or F. rodentium‐treated irradiated mice were examined by TUNEL staining.
-
PhytomedicineJieyu Guben decoction alleviates combined allergic rhinitis and asthma syndrome by balancing Th17/Treg expression and restoring PPARD. [Abstract]2025 Apr:139:156508. PMID: 40031093
One Step TUNEL Apoptosis Detection Kit (Cyanine 3) purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Apr:139:156508. [Abstract]
Cell apoptosis after JYGBD treatment of T cells was examined using a TUNEL assay. T cells after JYGBD treatments were fixed with 4 % PFA for 30 min, treated with permeabilization solution for 5 min at room temperature, and incubated with 50 μl of TUNEL working solution at 37 °C in darkness for 1 h.
-
Acta BiomaterCellular senescence imaging and senolysis monitoring in cancer therapy based on a β-galactosidase-activated aggregation-induced emission luminogen. [Abstract]2024 Apr 15:179:340-353. PMID: 38556136
One Step TUNEL Apoptosis Detection Kit (Cyanine 3) purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2024 Apr 15:179:340-353. [Abstract]
TUNEL fluorescence imaging of QM-β-gal and TUNEL, as well as Hoechst stained nuclei in senescent MDA-MB-231 and HCT116 incubated with ABT263 or DMSO (0.1%) as control for 24 hours
One Step TUNEL Apoptosis Detection Kit (Cyanine 3) purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2024 Apr 15:179:340-353. [Abstract]
Apoptotic TUNEL fluorescence with QM-β-gal and Hoechst in DOX+ABT263 group.
-
Clin Transl MedSpatiotemporal mapping reveals Ccl8hi macrophages as key drivers of testicular inflammaging. [Abstract]2025 Nov;15(11):e70527. PMID: 41251087
One Step TUNEL Apoptosis Detection Kit (Cyanine 3) purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2025 Nov;15(11):e70527. [Abstract]
Immunofluorescence staining of seminiferous tubules for TUNEL after 48 h of ex vivo culture with or without recombinant CCL8.
One Step TUNEL Apoptosis Detection Kit (Cyanine 3) purchased from MedChemExpress. Usage Cited in: Clin Transl Med. 2025 Nov;15(11):e70527. [Abstract]
Representative images and quantification of TUNEL staining in testes post‐injection.
-
Cell RepPodocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses. [Abstract]2024 May 16;43(5):114249. PMID: 38758648
-
Cancer Res CommunMutant Isocitrate Dehydrogenase 1 Sensitizes Intrahepatic Cholangiocarcinoma Cells to MDM2 Inhibitors. [Abstract]2026 Mar 1;6(3):616-629. PMID: 41747217
One Step TUNEL Apoptosis Detection Kit (Cyanine 3) purchased from MedChemExpress. Usage Cited in: Cancer Res Commun. 2026 Mar 1;6(3):616-629. [Abstract]
TUNEL staining in two IDH1mut iCCA cell lines after MDM2 inhibitors treatment for 1 doubling time. Blue staining represents DAPI. Red staining represents TUNEL positive cells. The white arrows point to TUNEL-positive/DAPI colocalized cells. IDH1 mutation iCCA cell lines: RBE and SNU1079. IDH1 wild-type iCCA cell lines: HuCCT1, HuH28, and SSP25. mIDH1 inhibitors: AG-120 and IDH-305. MDM2 inhibitors: RG7388 and HDM201.
Storage
-20°C, 1 year.
Protect from light.
Components
| Components | HY-K1079-20T | HY-K1079-50T |
|---|---|---|
| TdT Enzyme | 100 μL | 250 μL |
| Cy3-dUTP Labeling Mix | 900 μL | 2×1.2 mL |
| TdT Dilution Buffer | 300 μL | 1 mL |
Please enter the correct Lot Number
