2. MCE Kits
  3. Cell Biology
  4. Cell Analysis
  5. Cell Apoptosis and Necrosis
  6. One Step TUNEL Apoptosis Detection Kit (Cyanine 3)

One Step TUNEL Apoptosis Detection Kit (Cyanine 3) 

Cat. No.: HY-K1079
Manual SDS

MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. After staining cells with this kit, live cells have no fluorescence, apoptosis cells show red fluorescence.

One Step TUNEL Apoptosis Detection Kit (Cyanine 3)
Size Price Stock Quantity
20 T USD 425 In-stock
50 T USD 852 In-stock
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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE One Step TUNEL Apoptosis Detection Kit (Cyanine 3) provides a rapid and convenient method to detect cell apoptosis. When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180-200 bp fragments. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope or flow cytometer. The maximum excitation wavelength and emission wavelength of Cy3 (Cyanine 3) were 550 nm and 570 nm respectively.

Publications
Storage

-20°C, 1 year

Protect from light
Components
Components HY-K1079-20T HY-K1079-50T
TdT Enzyme 100 μL 250 μL
Cy3-dUTP Labeling Mix 900 μL 2×1.2 mL
TdT Dilution Buffer 300 μL 1 mL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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One Step TUNEL Apoptosis Detection Kit (Cyanine 3)
Cat. No.:
HY-K1079
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