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Cleaved-Caspase 3 p12 Antibody (YA499)

(Synonyms: CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1)
8 Cited Publications
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Based on 8 publication(s) in Google Scholar

Cleaved-Caspase 3 Antibody (YA499) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Cleaved-Caspase 3 p12 Antibody (YA499) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Publications Citing Use of MedChemExpress (MCE) Cleaved-Caspase 3 p12 Antibody (YA499)

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8 Publications Citing Use of MCE Cleaved-Caspase 3 p12 Antibody (YA499) (4)
WBIHC
  • IHC
  • WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Cleaved-Caspase 3 Antibody (YA499) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 32, 12 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 32 kDa
Species Reactivity Database

Entrez Gene: 836 Human

SwissProt: P42574 Human

Immunogen

Synthetic peptide corresponding to Human Caspase-3 p12.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3669005

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    Cleaved-Caspase 3 p12 is a Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively). Cleaves and inactivates interleukin-18 (IL18). Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:It is highly expressed in the lungs, spleen, heart, liver, and kidneys. It is moderately expressed in the brain and skeletal muscle, and lowly expressed in the testes. It is also present in many cell lines, with the highest expression in immune system cells.

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce

  • SwissProt ID

    P42574

  • Gene ID
    836 [NCBI]
  • Synonyms

    CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1

  • Research Field

    Cell Biology

Cleaved-Caspase 3 p12 Antibody (YA499) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: Nat Metab. 2026 Mar 5. [Abstract]

    Customer Validation
     Mediobasal hypothalamic sections (containing 3V tanycytes) from Rab27a KD and Con were immunostained for cleaved caspase 3.

  • 1/16

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: Exp Eye Res. 2025 Feb:251:110244. [Abstract]

    Customer Validation
     The western blot analysis demonstrated an elevated protein ratio of B-cell lymphoma 2 (Bcl-2) to Bax and an increased level of cleaved-Caspase-3 after AOH injury. Notably, these effects were significantly attenuated following TAT-N24 treatment.

  • 1/16

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: Theriogenology. 2025 Mar 15:235:121-133. [Abstract]

    Customer Validation
     The proapoptotic function of Caspase 3 usually relies on its activated protein forms, specifically cleaved Caspase 3 (CC3). CC3 protein levels were assessed in GC-2 cells treated with miR-143 inhibitor or mimics using WB.

  • 1/16

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 May 17;22(1):469. [Abstract]

    Customer Validation
     lncRNA RP11-197K6.1 knockdown increased the levels of apoptosis-related proteins, including cleaved caspase 3, caspase 7, and cleaved caspase 9, thus suggesting the activation of intrinsic apoptotic pathways. Notably, no significant change was observed in the expression level of cleaved caspase 8.

  • Cleaved-Caspase 3 p12 Antibody (YA499) Product Image
    1/16
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Experimental Validation Results for Cleaved-Caspase 3 p12 Antibody (YA499)
  • Cleaved-Caspase 3 p12 Antibody (YA499) Product Image

  • Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: Nat Metab. 2026 Mar 5. [Abstract]

    Mediobasal hypothalamic sections (containing 3V tanycytes) from Rab27a KD and Con were immunostained for cleaved caspase 3.

  • Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: Exp Eye Res. 2025 Feb:251:110244. [Abstract]

    The western blot analysis demonstrated an elevated protein ratio of B-cell lymphoma 2 (Bcl-2) to Bax and an increased level of cleaved-Caspase-3 after AOH injury. Notably, these effects were significantly attenuated following TAT-N24 treatment.

  • Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: Theriogenology. 2025 Mar 15:235:121-133. [Abstract]

    The proapoptotic function of Caspase 3 usually relies on its activated protein forms, specifically cleaved Caspase 3 (CC3). CC3 protein levels were assessed in GC-2 cells treated with miR-143 inhibitor or mimics using WB.

  • Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 May 17;22(1):469. [Abstract]

    lncRNA RP11-197K6.1 knockdown increased the levels of apoptosis-related proteins, including cleaved caspase 3, caspase 7, and cleaved caspase 9, thus suggesting the activation of intrinsic apoptotic pathways. Notably, no significant change was observed in the expression level of cleaved caspase 8.