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  5. Cleaved-Caspase 3 p12 Antibody (YA499)

Cleaved-Caspase 3 p12 Antibody (YA499)

Cat. No.: HY-P80623
COA
SDS
User Guide for Antibodies Technical Support

Cleaved-Caspase 3 Antibody (YA499) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: Nat Metab. 2026 Mar 5.

    Mediobasal hypothalamic sections (containing 3V tanycytes) from Rab27a KD and Con were immunostained for cleaved caspase 3.

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: Exp Eye Res. 2025 Jan 18:110244.  [Abstract]

    The western blot analysis demonstrated an elevated protein ratio of B-cell lymphoma 2 (Bcl-2) to Bax and an increased level of cleaved-Caspase-3 after AOH injury. Notably, these effects were significantly attenuated following TAT-N24 treatment.

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: Theriogenology. 2025 Mar 15:235:121-133.

    The proapoptotic function of Caspase 3 usually relies on its activated protein forms, specifically cleaved Caspase 3 (CC3). CC3 protein levels were assessed in GC-2 cells treated with miR-143 inhibitor or mimics using WB.

    Cleaved-Caspase 3 p12 Antibody (YA499) purchased from MCE. Usage Cited in: J Transl Med. 2024 May 17;22(1):469.  [Abstract]

    lncRNA RP11-197K6.1 knockdown increased the levels of apoptosis-related proteins, including cleaved caspase 3, caspase 7, and cleaved caspase 9, thus suggesting the activation of intrinsic apoptotic pathways. Notably, no significant change was observed in the expression level of cleaved caspase 8.
    • WB: Western Blot;
    • IHC-P: Immunohistochemistry-Paraffin;
    • IHC-F: Immunohistochemistry-Frozen;
    • ICC/IF: Immunocytochemistry/Immunofluorescence;
    • IF-Tissue: Immunofluorescence-Tissue;
    • mIHC: Multiplex Immunohistochemical;
    • IP: Immunoprecipitation;
    • ChIP: Chromatin Immunoprecipitation;
    • FC: Flow Cytometry;
    • ELISA: Enzyme Linked Immunosorbent Assay

    • Product Detail

    • Background

    • Documentation

    Description

    Cleaved-Caspase 3 Antibody (YA499) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cleaved-Caspase 3.
    Host

    Rabbit
    Clonality

    Recombinant,Monoclonal
    Molecular Weight
    Predicted band size: 32 kDa;
    Observed band size: 32, 12 kDa
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
    Species Reactivity
    Human, Mouse, Rat
    SwissProt ID
    Gene ID
    Immunogen

    Synthetic peptide corresponding to Human Caspase-3 p12.The exact sequence is proprietary to MCE.
    Application &
    Dilution Ratio
    Application Dilution Ratio
    WB
    WB: Western Blot
    		1:500-1:1000
    ICC/IF
    ICC/IF: Immunocytochemistry/Immunofluorescence
    		1:50-1:200
    Sensitivity Endogenous Purity affinity purified
    Conjugation Non-conjugated Modification Unmodified
    Isotype IgG  
    Appearance

    Liquid
    Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide
    Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
    Shipping

    Shipping with blue ice.
    Background
    Function：Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:35338844, PubMed:35446120, PubMed:7596430). Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins (PubMed:18723680, PubMed:20566630, PubMed:23650375, PubMed:7596430). At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond (PubMed:10497198, PubMed:16374543, PubMed:7596430, PubMed:7774019). Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain (By similarity). Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively) (PubMed:7596430). Cleaves and inactivates interleukin-18 (IL18) (PubMed:37993714, PubMed:9334240). Involved in the cleavage of huntingtin (PubMed:8696339). Triggers cell adhesion in sympathetic neurons through RET cleavage (PubMed:21357690). Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress (PubMed:23152800). Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction (PubMed:30878284). Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME) (PubMed:35338844, PubMed:35446120). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:33725486). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758104, PubMed:36758106)
    Subcellular Localization：Cytoplasm
    Expression：
    Tissue_specificity:It is highly expressed in the lungs, spleen, heart, liver, and kidneys. It is moderately expressed in the brain and skeletal muscle, and lowly expressed in the testes. It is also present in many cell lines, with the highest expression in immune system cells.
    Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce
    RRID
    Database

    Entrez Gene: 836 Human

    SwissProt: P42574 Human

    OMIM: 600636 Human

    Research Field

    Cell Biology
    Synonyms
    CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA-1
    Documentation
    SDS (252 KB)

    COA (206 KB)

    User Guide for Antibodies (1077 KB)

    Cleaved-Caspase 3 p12 Antibody (YA499) Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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