IMDM (Iscove's Modified Dulbecco's Medium)
MCE IMDM (Iscove's Modified Dulbecco's Medium) is an enriched formulation modified from DMEM, containing high concentrations of glucose, sodium pyruvate, amino acids, vitamins, HEPES buffer, selenium, and other essential nutrients. It is designed to meet the nutritional requirements for rapid cell proliferation and high-density culture, supporting a variety of cell types, including Jurkat cells, COS-7 cells, and macrophages. The product specifications are based on the 500 mL format, and larger volume packages are provided as multiples of this standard specification.
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Storage :
2–8°C, 1 year. Protect from light.
- Manual (1934KB)
- COA
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Description & Advantages
MCE IMDM medium is an enriched formulation modified from DMEM, containing high concentrations of glucose, sodium pyruvate, amino acids, vitamins, HEPES buffer, selenium, and other essential nutrients. It is designed to meet the nutritional requirements for rapid cell proliferation and high-density culture, supporting a variety of cell types, including Jurkat cells, COS-7 cells, and macrophages.
This product is suitable for the culture of various hematopoietic and immune cell types, including mouse B lymphocytes, bone marrow-derived hematopoietic cells, lipopolysaccharide (LPS)-stimulated B cells, and T lymphocytes, as well as diverse mixed cell populations. When supplemented with serum, this medium can also support a broad range of mammalian cell types, including but not limited to bone marrow cells, hybridoma cells, and lymphocytes.
Protocols
| With(+) | With(-) |
|---|---|
| D-Glucose (4.5 g/L) | α-Thioglycerol |
| Phenol Red (15 mg/L) | β-Mercaptoethanol |
| L-Glutamine (4 mM) | |
| Sodium Pyruvate | |
| HEPES (25 mM) |
IMDM is a basal culture medium and does not contain proteins, lipids, or growth factors. Appropriate supplements should be added according to the cell type and experimental requirements. For routine culture, approximately 10% fetal bovine serum may be added. For low-serum or serum-free culture systems, supplements such as Insulin-Transferrin-Selenium (ITS) (HY-K1018) may be added as needed.
This product contains L-glutamine. L-glutamine gradually degrades during storage in liquid culture media. Therefore, if the medium is stored at 2-8°C for an extended period, supplementation with additional L-glutamine is recommended according to cell requirements and culture performance.
Note: This product does not contain antibiotics. If required, Penicillin-Streptomycin Solution (HY-K1006) may be added according to experimental requirements. After supplementation with serum, ITS, antibiotics, or other components, the prepared medium should be stored at 2-8°C and used within 1 week.
Storage
2–8°C, 1 year.
Protect from light.
Attention
1. This product has been sterilized by filtration. Use aseptic techniques during handling to prevent microbial contamination.
2. For cell culture applications, cells should be maintained in a humidified incubator at the appropriate temperature with 5%-10% CO2, as required for the specific cell type and culture conditions.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K3036-500 mL | HY-K3036-1 L | HY-K3036-3 L | HY-K3036-5 L |
|---|---|---|---|---|
| IMDM (Iscove's Modified Dulbecco's Medium) | 500 mL | 500 mL × 2 | 500 mL × 6 | 500 mL × 10 |
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