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  3. Cell Biology
  4. Cell Analysis
  5. Metabolism Assay Kit
  6. NAD+/NADH Assay Kit (WST-8)

NAD+/NADH Assay Kit (WST-8) 

Cat. No.: HY-K0313
Manual COA SDS Technical Support

MCE NAD+/NADH Assay Kit (WST-8) is a colorimetric assay that conveniently detects the oxidized (NAD+) and reduced (NADH) forms of coenzyme Ⅰ within cells, tissues, and other samples.

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Bio/Physico-chemical Assay

    NAD+/NADH Assay Kit (WST-8) purchased from MCE. Usage Cited in: Cell Death Dis. 2025 Nov 7;16(1):810.  [Abstract]

    The relative NAD+/NADH ratio in ESCC cell lines was quantified by enzymatic assay.

    NAD+/NADH Assay Kit (WST-8) purchased from MCE. Usage Cited in: J Agric Food Chem. 2025 Aug 13;73(32):20385-20395.  [Abstract]

    PKM2 was a key enzyme regulated by ZEN to inhibit glycolysis and cell proliferation in goat endometrial epithelial cells. The NAD+/NADH ratio was altered. Cells were lysed in cold extraction buffer (10 min) and centrifuged (12 000g, 10 min, 4 °C), and supernatants were incubated (60 °C, 30 min) to hydrolyze NAD+. NAD+ and NADH concentrations were determined per the manufacturer's instructions, and their ratio was calculated.

    • Description

    • Storage

    • Components

    • Documentation

    Description
    & Advantages

    MCE NAD+/NADH Assay Kit (WST-8) is a colorimetric assay that conveniently detects the oxidized (NAD+) and reduced (NADH) forms of coenzyme Ⅰ within cells, tissues, and other samples. This is achieved through colorimetric measurements based on the WST-8 color reaction, eliminating the need for purification of NAD+/NADH from the samples.
    Storage

    -20℃, 1 year

    Keep away from light and avoid repeat freeze-thaw cycles
    Components
    Components HY-K0313-100 T
    NAD+/NADH Extract Solution 50 mL
    NADH 0.5 mg
    NADH Solution 1 mL
    ADH 500 μL
    Chromogen Solution 1.1 mL
    Reaction Buffer 10 mL
    Documentation

    Manual (4179 KB) SDS (4595 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Total NAD and NADH Fluorimetric Detection Assay KitNAD+/NADH Assay Kit with WST-8Redox Assay

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