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Paclitaxel 

Cat. No.: HY-B0015 Purity: 99.60%
Data Sheet SDS Handling Instructions

Paclitaxel is a potent anticancer agent known to promote microtubule (MT) assembly, inhibit MT depolymerization, and change MT dynamics required for mitosis and cell proliferation.

For research use only. We do not sell to patients.
Paclitaxel Chemical Structure

Paclitaxel Chemical Structure

CAS No. : 33069-62-4

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $55 In-stock
50 mg $50 In-stock
100 mg $80 In-stock
500 mg $120 In-stock
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    Paclitaxel purchased from MCE. Usage Cited in: Am J Cancer Res. 2017 Apr 1;7(4):903-912.

    Synergistic inhibition of colony formation of ATC cells by Lenvatinib and Paclitaxel. Representative images of colony formation in C643 cells treated with vehicle control (DMSO) or Lenvatinib and Paclitaxel at the indicated concentrations, individually or in combination, are shown in left panel. Quantitative analysis of colony numbers is shown in right panel.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    Paclitaxel is a potent anticancer agent known to promote microtubule (MT) assembly, inhibit MT depolymerization, and change MT dynamics required for mitosis and cell proliferation.

    IC50 & Target

    IC50: 4 nM (MT)

    In Vitro

    Paclitaxel at 0.1, 0.5, and 1 μM reduces the proliferation and survival of CCRF-HSB-2 cells in a dose-dependent fashion and that the IC50 value of taxol is about 0.25 μM[1]. Paclitaxel directly associates with the endoplasmic reticulum to stimulate the release of calcium into the cytosol, contributing to the induction of apoptosis[2].

    In Vivo

    In a SCID mouse xenograft model, low dose metronomic Paclitaxel treatment decreases lung dissemination of EGI-1 cells without significantly affecting their local tumor growth[3]. Low doses of paclitaxel promot liver metastasis in mouse xenografts, which correlats with changes in estrogen metabolism in the host liver[4]. Paclitaxel (2 mg/kg per treatment, black circles) induces mechanical hypersensitivity in the glabrous skin of the hindpaw[5].

    Clinical Trial
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    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 1.1711 mL 5.8554 mL 11.7108 mL
    5 mM 0.2342 mL 1.1711 mL 2.3422 mL
    10 mM 0.1171 mL 0.5855 mL 1.1711 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay
    [1]

    To determine which caspases are involved in apoptosis induced by taxol, caspase-3 inhibitor (DEVD-CHO), caspase-6 inhibitor (Z-VEID-FMK), caspase-8 inhibitor (Z-IETD-FMK or IETD-CHO), caspase-9 inhibitors (Z-LEHD-FMK or LEHD-CHO), and caspase-10 inhibitor (Z-AEVD-FMK) are used. These caspase inhibitors are dissolved in dimethyl sulfoxide (Me2SO); the final concentration of Me2SO is 0.1%. Cells (5×105) are preincubated in the presence or absence of 100 μM each of these inhibitors for 3 h at 37°C then treated with or without 0.1, 0.5, and 1 μM Paclitaxel for 48 h and processed for annexin V binding assay. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Assay
    [1]

    1×104 cells are plated in 100 μL of the growth medium in the presence or absence of increasing concentrations (0.1-1 μM) of taxol in 96-well plates and cultured at 37°C in 5% CO2 for 12-48 h. The cells are then incubated with 25 μL of MTT (5 mg/mL) at 37°C for 4 h. After dissolving the crystals with 0.04 N HCl in isopropanol, the plates are read in a microplate reader at 570 nm. The concentration of drug that inhibits cell survival by 50% (IC50) is determined from cell survival plots. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [5]

    Paclitaxel is dissolved at 25 mg/mL in 1:1 Cremophor EL: ethanol and freshly diluted 1:12.5 in 0.9% sterile saline prior to injections.

    Adult (250-320 g) male Sprague-Dawley rats are used for all experiments. Rats are housed two per cage in a temperature and humidity controlled, on a 12 h:12 h light:dark schedule with food and water available ad libitum. One week following the DiI injection, rats are anesthetized with isofluorane and injected into the tail vein with 2 mg/kg paclitaxel or its vehicle (1:1:23, cremophor EL:ethanol:0.9% saline). The tail vein injection is repeated three more times every other day for a total of four injections. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    853.91

    Formula

    C₄₇H₅₁NO₁₄

    CAS No.

    33069-62-4

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    DMSO: ≥ 31 mg/mL; H2O: < 0.1 mg/mL

    Paclitaxel is dissolved at 20 mg/mL in DMSO and freshly diluted (1:1-1:200) as a suspension with PBS.
    Paclitaxel is dissolved in DMSO, aliquoted and stored at -80°C until further use. The same volume of DMSO is used as the vehicle control[6].

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

    References

    Purity: 99.60%

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    Inquiry Information

    Product Name:
    Paclitaxel
    Cat. No.:
    HY-B0015
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