NADH disodium salt
Based on 8 publication(s) in Google Scholar
NADH disodium salt (Disodium NADH) is an orally active reduced coenzyme. NADH disodium salt is a donor of ADP-ribose units in ADP-ribosylaton reactions and a precursor of cyclic ADP-ribose. NADH disodium salt plays a role as a regenerative electron donor in cellular energy metabolism, including glycolysis, β-oxidation and the tricarboxylic acid (TCA) cycle.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 606-68-8
- Formula: C21H27N7Na2O14P2
- Molecular Weight:709.40
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) NADH disodium salt
More- Cell Mol Immunol. 2024 Jun;21(6):561-574. [Abstract]
- Food Chem. 2023 Oct 15:423:136274. [Abstract]
- Free Radic Biol Med. 2025 Jan:226:56-69. [Abstract]
- J Ginseng Res. 2025 Sep;49(5):541-552. [Abstract]
- Mol Plant Pathol. 2025 Dec;26(12):e70196. [Abstract]
- Biochemistry. 2023 Dec 5;62(23):3396-3410. [Abstract]
- Chemrxiv. 2025 Aug 28.
- Research Square Preprint. 2023 Sep 15.
All Endogenous Metabolite Isoforms
More
Biological Activity
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Human Endogenous Metabolite |
NADH is unstable under acidic conditions but it is stable under alkaline conditions[2].
NADH (0-1 mM; 0-12 h) increases NAD+ levels in various mammalian cell lines[3].
NADH (1 mM; 24 h) causes low toxicity and protects cells from genotoxicity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
NADH (500 mg/kg; i.g.; once) promotes alcohol metabolism and prevents or ameliorates early liver injury caused by acute alcohol exposure in ethanol-loaded mice[3].
NADH (1000 mg/kg; i.p.; once) enhances tissue NAD+ levels in male C57BL/6J mice[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male ICR mice[2]
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Dosage:5 μmol/mouse
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Administration:Intraperitoneal injection or oral administration, once
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Result:Produced significant increases in urinary excretions of nicotinamide (Nam) with intraperitoneal injection. Oral administration did not produce any increases in Nam or its metabolites.
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Animal Model:Male C57BL/6J mice[3]
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Dosage:500 mg/kg
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Administration:Intragastric administration, 15 min before ethanol administration
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Result:Significantly increased blood acetaldehyde levels in mice administered with alcohol between 30 min and two hours. Significantly reduced the acetaldehyde in the blood after two hours. Inhibited the decrease of NAD+/NADH redox ratio in hepatocytes.
Chemical Information
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CAS No. 606-68-8
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Appearance Solid
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Molecular Weight 709.40
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Formula C21H27N7Na2O14P2
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Color Off-white to yellow
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C=C(C(N)=O)CC=C2)O[C@@H]1COP(OP(OC[C@@H]3[C@@H](O)[C@@H](O)[C@H](N(C4=NC=N5)C=NC4=C5N)O3)(O[Na])=O)(O[Na])=O
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Synonyms
Disodium NADH
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (8)
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Journal Impact Factor
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Most Recent
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Cell Mol Immunol
2024 Jun;21(6):561-574. PMID: 38570588 -
Food Chem
Improvement of catalytic activity of sorbose dehydrogenase for deoxynivalenol degradation by rational design. [Abstract]2023 Oct 15:423:136274. PMID: 37159968 -
Free Radic Biol Med
TSPO exacerbates sepsis-induced cardiac dysfunction by inhibiting p62-Mediated autophagic flux via the ROS-RIP1/RIP3-exosome axis. [Abstract]2025 Jan:226:56-69. PMID: 39542185 -
J Ginseng Res
Total ginsenosides and ginsenoside Rb2 delay hepatocyte senescence by regulating NAD+ metabolism and promoting IDO2/QPRT expression. [Abstract]2025 Sep;49(5):541-552. PMID: 40843012 -
Mol Plant Pathol
Negative Immune Regulator CAD7 Functions as a Small-Molecule Aldehyde Reductase and Increases Histamine Accumulation in Arabidopsis. [Abstract]2025 Dec;26(12):e70196. PMID: 41456913 -
Biochemistry
Full-Length NAD+-I Riboswitches Bind a Single Cofactor but Cannot Discriminate against Adenosine Triphosphate. [Abstract]2023 Dec 5;62(23):3396-3410. PMID: 37947391 -
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Solvent & Solubility
H2O : 100 mg/mL (140.96 mM; Need ultrasonic)
DMSO : 100 mg/mL (140.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.52 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.52 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (286 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Ying W. NAD+ and NADH in cellular functions and cell death. Front Biosci. 2006 Sep 1;11:3129-48. [Content Brief]
[2]. Kimura N, et al. Comparison of metabolic fates of nicotinamide, NAD+ and NADH administered orally and intraperitoneally; characterization of oral NADH. J Nutr Sci Vitaminol (Tokyo). 2006 Apr;52(2):142-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 1.4096 mL | 7.0482 mL | 14.0964 mL | 35.2410 mL |
| 5 mM | 0.2819 mL | 1.4096 mL | 2.8193 mL | 7.0482 mL | |
| 10 mM | 0.1410 mL | 0.7048 mL | 1.4096 mL | 3.5241 mL | |
| 15 mM | 0.0940 mL | 0.4699 mL | 0.9398 mL | 2.3494 mL | |
| 20 mM | 0.0705 mL | 0.3524 mL | 0.7048 mL | 1.7621 mL | |
| 25 mM | 0.0564 mL | 0.2819 mL | 0.5639 mL | 1.4096 mL | |
| 30 mM | 0.0470 mL | 0.2349 mL | 0.4699 mL | 1.1747 mL | |
| 40 mM | 0.0352 mL | 0.1762 mL | 0.3524 mL | 0.8810 mL | |
| 50 mM | 0.0282 mL | 0.1410 mL | 0.2819 mL | 0.7048 mL | |
| 60 mM | 0.0235 mL | 0.1175 mL | 0.2349 mL | 0.5874 mL | |
| 80 mM | 0.0176 mL | 0.0881 mL | 0.1762 mL | 0.4405 mL | |
| 100 mM | 0.0141 mL | 0.0705 mL | 0.1410 mL | 0.3524 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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