Lentivirus Packaging Kit
Based on 1 Customer Validation
MCE Lentivirus Packaging Kit is based on the second-generation lentiviral packaging system and is also compatible with both second- and third-generation packaging plasmids. Lentiviruses produced using this kit exhibit high infection efficiency and a broad host range, enabling stable and sustained expression of exogenous genes in host cells.
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Storage :
-20°C, 1 year; 4°C, 6 months.
Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
The lentiviral vector is a gene therapy vehicle derived from HIV-1, capable of stably integrating exogenous genes into the host cell genome. It features a broad host range, enables efficient infection of both dividing and non-dividing cells, and ensures long-term and stable expression of transgenes. Therefore, it has become a powerful tool for gene delivery, widely used in gene overexpression, RNA interference, microRNA studies, and in vivo animal experiments across various cell lines.
MCE Lentivirus Packaging Kit is based on the second-generation lentiviral packaging system and is also compatible with both second- and third-generation packaging plasmids. Lentiviruses produced using this kit exhibit high infection efficiency and a broad host range, enabling stable and sustained expression of exogenous genes in host cells.
Storage
-20°C, 1 year; 4°C, 6 months.
Avoid repeated freeze-thaw cycles.
Attention
1. Avoid repeated freeze/thaw cycles of plasmids. It is recommended to aliquot plasmids according to experimental needs and store them at -20°C for long-term preservation to ensure plasmid purity and transfection efficiency.
2. When collecting crude viral supernatant, in addition to routine aliquoting, it is advisable to prepare several small-volume aliquots (20-100 μL). Store these together with the main viral stock at -80°C. Once the small aliquots are fully frozen, thaw one aliquot and perform a titer determination. This simulates the titer reduction caused by a single freeze/thaw cycle, providing a more realistic and representative viral titer value that better reflects the actual infection efficiency.
3. All lentivirus-related procedures—including transfection, viral harvest, infection, and waste disposal—must be conducted in a BSL-2 (biosafety level 2) biosafety cabinet to ensure safe handling and containment.
4. This product is for R&D use only, not for drug, household, or other uses.
5. For your safety and health, please wear a lab coat and disposable gloves to operate.
Components
| Components | HY-K2030-10 T | HY-K2030-20 T | HY-K2030-40 T |
|---|---|---|---|
| Lentiviral Packaging Plasmid Mix | 40 μL | 80 μL | 160 μL |
| Control Plasmid (1 μg/μL) | 10 μL | 20 μL | 40 μL |
| Lentivirus Transfection Reagent | 400 μL | 800 μL | 1600 μL |
| Polybrene (10 mg/mL) | 200 μL | 400 μL | 800 μL |
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