Basement Membrane Matrix GFR&HC 

•   MCE Basement Membrane Matrix GFR&HC is a natural basement membrane matrix extracted from mouse tumors and is composed mainly of various growth factors and extracellular matrix components. The main extracellular matrix components are: Laminin, Col-IV, Entactin, Heparan sulphate proteoglycans, etc. The main growth factor components are: insulin-like growth factor (IGF-1), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), fibroblast growth factor (bFGF), etc. The percentage of each of these growth factors is lower than that of standard basement membrane matrix.

•   MCE Basement Membrane Matrix GFR&HC reduces the background signal induced by growth factors and is suitable for cell cultures that need to reduce growth factor induction and studies related to growth factors, signaling pathways, etc.

-20°C, 2 years.

Avoid repeated freezing and thawing.

a. Use a pre-cooled pipette to blow the matrix gel evenly after thawing.

b. Dilute MCE Basement Membrane Matrix GFR&HC with cell suspension at 4°C in a 1:1 ratio to a suitable final concentration.

c. Inject diluted cell suspension at a depth of 1 cm under the skin on nude mice. The cell suspension should be injected in 30 minutes and be placed on ice to slow down apoptosis and prevent from gelling.

d. Formed tumors can be seen in about 1 week.