TAE Powder (1 L of 1×) 

TAE (Tris-acetate-EDTA) buffer is widely used in biological experiments, especially in agarose gel electrophoresis of nucleic acid. Linear dsDNA migrates quickly in electrophoresis and DNA fragments (> 13 kb) can be separated effectively. TAE buffer is also suitable for DNA gel extraction.

MCE TAE Powder (1 L of 1×) consists of Tris-acetate and EDTA-2Na. Each pouch can be diluted to 1 L of 1× TAE buffer, in which the concentration of Tris-acetate is 40 mM and the concentration of EDTA-2Na is 1 mM. This product provides a convenient way to make TAE solution and eliminates the need to weigh and mix individual components.

Store at room temperature for 3 years.

1. Add 1 pouch of TAE Powder into the cleaned beaker, dissolve with 600 mL distilled water under a magnetic stirrer.

2. Add distilled water to the solution in step 1 until the total volume is 1 L. The final solution is 1× TAE buffer.

Note: The pH of the 1× TAE buffer is 8.3 ± 0.1 @ 25°C.