1. MCE Kits
  2. Molecular Biology
  3. PCR & qPCR
  4. DNA Polymerase
  5. Phi29 DNA Polymerase(mutant)

Phi29 DNA Polymerase(mutant) 

Cat. No.: HY-KE8011
Manual SDS

Phi29 DNA polymerase is a protein-engineered Phi29 DNA polymerase expressed in E. coli and then purified and isolated multiple times. Compared with wild-type Phi29 DNA polymerase, it has higher amplification efficiency and sensitivity, and can greatly shorten the reaction time.

Phi29 DNA Polymerase(mutant)
Size Price Stock
125 U USD 38 Ask For Quote & Lead Time

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

Phi29 DNA polymerase is a protein-engineered Phi29 DNA polymerase expressed in E. coli and then purified and isolated multiple times. Compared with wild-type Phi29 DNA polymerase, it has higher amplification efficiency and sensitivity, and can greatly shorten the reaction time. Phi29 DNA polymerase (mutant) has special strand displacement activity and efficient continuous synthesis characteristics. It has strong binding ability to templates and can continuously synthesize DNA fragments up to 70kb without dissociating from the template. At the same time, this enzyme has a strong 3´→5´ exonuclease proofreading function, and its fidelity is 100 times higher than Taq DNA polymerase. This product has been column purified multiple times. Only a clear single purpose band can be seen in SDS-PAGE gel detection. There is no E. coli DNA residue and no endonuclease or exonuclease contamination in qPCR detection.

Storage

-20ºC, year

Components
Components HY-KE8011-125 U
Phi29 DNA Polymerase(mutant) (10 U/μL) 12.5 μL
10× Phi29 Buffer 1 mL
100× BSA 200 μL
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phi29 DNA Polymerase(mutant)
Cat. No.:
HY-KE8011
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