1. MCE Kits
  2. Molecular Biology
  3. Nucleic Acid Gel Electrophoresis
  4. Nucleic Acid Gel Stain
  5. SYBR Green I Nucleic Acid Gel Stain

SYBR Green I Nucleic Acid Gel Stain 

Cat. No.: HY-K1004
Manual SDS

SYBR Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels.

SYBR Green I Nucleic Acid Gel Stain
Size Price Stock Quantity
Free Sample (50 μL)   Apply Now  
100 μL USD 55 In-stock
500 μL USD 170 In-stock

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

& Advantages

SYBR Green I Nucleic Acid Gel Stain is a proprietary unsymmetrical cyanine dye that has proven exceptionally useful for assays requiring sensitive nucleic acid detection. SYBR Green I stain is maximally excited at 490 nm and has secondary excitation peaks at 290 nm and 380 nm. The fluorescence emission of SYBR Green I Stain bound to DNA is centered at 520 nm. The detection limit using SYBR Green I Stain is as low as 60 pg per band of dsDNA using 300 nm transillumination. With 254 nm epi-illumination, as little as 20 pg of dsDNA can be detected. SYBR Green I Stain is also possible to stain dsDNA prior to electrophoresis as it has an exceptionally high affinity for dsDNA.


•   High sensitivity: Detect as little as 20 pg dsDNA per band

•   High signal-to-noise ratio: strong fluorescence signal of the sample, low background signal

•   Strong applicability: suitable for agarose gel or polyacrylamide gel electrophoresis; used for dsDNA, ssDNA or RNA staining

•   No destaining or washing steps, compatible with different testing equipments


Stored at -20°C, and is stable for 1 year.

Protect from light.


Staining DNA Before Electrophoresis

a.   Precast agarose or nondenaturing polyacrylamide gels (0.8-3.0%) with SYBR Green I stain by diluting the SYBR Green I stock reagent 1:10,000 into the gel solution just prior to pouring the gel.

b.   Mix SYBR Green I stain with 6× Loading Buffer at a ratio of 1:9 to get Loading Buffer pre-dye. Then mix the nucleic acid sample and marker with Loading Buffer pre-dye at a ratio of 5:1 respectively and incubate at room temperature for at least 15 minutes prior to electrophoresis.

Note: Loading Buffer containing SDS would affect the electrophoresis results. It is recommended to use a Loading Buffer without SDS.

c.   After loading and electrophoresis, detect the bands under UV illuminator.

Staining DNA Following Electrophoresis

a.   Perform electrophoresis on an agarose or nondenaturing polyacrylamide gel without stain.

b.   Dilute the stock SYBR Green I reagent with a buffer of pH 7.5-8.0 (such as TAE, TBE or TE) at a certain ratio (the ratio between dye and agarose gel is 1:10,000 or 2:10,000; the ratio between dye and polyacrylamide gel is 3:10,000 or 4:10,000) and gently shake to form a staining solution.

c.   Cover the gel with staining solution in a plastic container and incubate at room temperature for 30–60 minutes with shaking. Protect the staining container from light by covering it with aluminum foil or placing it in the dark. Staining time varies depending on the thickness of the gel and the percentage of agarose or polyacrylamide.

d.   Detect the bands under UV illuminator.

Cat. No. Product Name Package
HY-K1004-100 μL SYBR Green I Nucleic Acid Gel Stain (10,000 ×) 100 μL
HY-K1004-500 μL SYBR Green I Nucleic Acid Gel Stain (10,000 ×) 500 μL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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