Seamless DNA Assembly Plus Kit
Based on 3 publication(s) in Google Scholar
MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.
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Storage :
Store at -20°C for 2 years. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Description & Advantages
Based on gene recombination, seamless cloning is a simple, efficient, and fast DNA cloning technology. A 15-20 bp sequence homologous to the end of the linearized vector is added to the 5′ end of the insert fragment primers. In the presence of enzymes, recombination can be easily achieved.
MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.
Publications
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Journal Impact Factor
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Most Recent
Storage
Store at -20°C for 2 years. Avoid repeated freeze-thaw cycles.
Components
| Components | HY-K1041-50 rxns |
|---|---|
| Seamless Assembly Mix Plus | 250 μL |
| pUC19 Control Plasmid, Linearized (Ampr, 40 ng/μL) | 5 μL |
| 500 bp Control Fragment (20 ng/μL) | 5 μL |
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