Seamless DNA Assembly Plus Kit

Cat. No.: HY-K1041: FAQs
Manual SDS

MCE Seamless DNA Assembly Plus Kit contains an optimized mix of recombinase, reaction buffer, and additional cofactors that significantly improve the cloning efficiency and tolerance to impurities. This product can complete multiple DNA fragments recombination and takes only 5 minutes for single fragment, and the positive rate is more than 95%.

Size	Price	Stock	Quantity
50 rxns	USD 170	In-stock	Estimated Time of Arrival: December 31

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Description
Storage
Components
Documentation

Description
& Advantages

Based on gene recombination, seamless cloning is a simple, efficient, and fast DNA cloning technology. A 15-20 bp sequence homologous to the end of the linearized vector is added to the 5´ end of the insert fragment primers. In the presence of enzymes, recombination can be easily achieved.

Storage

Store at -20°C for 2 years. Avoid repeated freeze-thaw cycles.

Components

Components	HY-K1041-50 rxns
Seamless Assembly Mix Plus	250 μL
pUC19 Control Plasmid, Linearized (Ampr, 40 ng/μL)	5 μL
500 bp Control Fragment (20 ng/μL)	5 μL

Documentation

Manual (729 KB) SDS (118 KB)

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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