SYBR Green qPCR Master Mix (High ROX) 

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer and ddH₂O.

MCE qPCR Master Mix (High ROX) can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. High ROX Reference Dye is included for use with instruments that require a high level of reference dye for rectify the error of fluorescence signals between different wells.

MCE qPCR Master Mix (High ROX) is applied for the Real-Time PCR instrument as follows:

ABI GeneAmp 5700; ABI PRISM 7000, 7700; ABI 7300, 7900HT (Fast); ABI StepOne (Plus)

1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. High ROX Reference Dye is included.

• •Acta Biomater. 2021 Mar 9;S1742-7061(21)00152-5.  [Abstract]
• •Cancer Cell Int. 2022 Jan 5;22(1):5.  [Abstract]

Stored at -20°C protecting from light, and is stable for up to 18 months. Avoid repetitive freeze-thaw cycles while using. For immediate use, components may be stored at 2-8°C.