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Agarose 

Cat. No.: HY-K1031
Manual SDS

MCE Agarose can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.

Agarose
Size Price Stock Quantity
100 g USD 60 In-stock
Estimated Time of Arrival: December 31

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Agarose is widely used for nucleic acid isolation and identification. This product can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.


Features
Gel Strength (1% gel) > 1,200 g/cm2
Electroosmosis (EEO) < 0.15
Sulfide ≤ 0.15%
Gel Point (1.5% gel) 35-37°C
Melting Point (1.5% gel) 87-89°C
Water concentration ≤ 10%
Nuclease None
Storage

Store at room temperature for 3 years.

Protocol

1.   Choose appropriate agarose concentration according to the size of nucleic acid fragment and the type of electrophoresis buffer.

2.   Prepare the gel

a.     Add appropriate agarose into the cleaned conical flask and dissolve with appropriate electrophoresis buffer (TAE or TBE). Seal the flask and heat in the microwave until the agarose is completely dissolved.

b.     Add Nucleic Acid dye (HY-K1004 MCE SYBR Green I Nucleic Acid Gel Stain).

c.     Mix gently and pour the agarose solution into the rubber board and insert the comb in place. The thickness of the gel is generally 3-5 mm.

d.     Cool at room temperature for 30-60 minutes for subsequent electrophoresis.

Components
Components HY-K1031-100 g
Agarose 100 g
Documentation

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Product Name:
Agarose
Cat. No.:
HY-K1031
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