1. MCE Kits
  2. Molecular Biology
  3. PCR & qPCR
  4. Real-Time PCR
  5. SYBR Green qPCR Master Mix

SYBR Green qPCR Master Mix 

Cat. No.: HY-K0501
Manual SDS

MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water.

SYBR Green qPCR Master Mix
Size Price Stock Quantity
Free Sample (50 rxns)   Apply Now  
100 rxns USD 40 In-stock
500 rxns USD 132 In-stock

* Please select Quantity before adding items.

Customer Validation

mRNA expression of AB, SO, CD, OC, NA and GP genes in H-GFP and H-5A cell lines assessed by RT-qPCR.

Relative expression of MDM2, p21, Survivin, PUMA, BAX and Bcl2 genes in N-3a treated cells compared to controls.

  • Description

  • Storage

  • Components

  • Documentation

& Advantages

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

MCE qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Two separate tubes of ROX Reference Dye are included for use with instruments that require a high or low level of reference dye for rectify the error of fluorescence signals between different wells.


1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. Passive reference dyes are provided for different qPCR instruments.


Stored at -20°C protecting from light, and is stable for up to 18 months. Avoid repetitive freeze-thaw cycles while using. For immediate use, components may be stored at 2-8°C.

Components HY-K0501-100rxns HY-K0501-500rxns
SYBR Green qPCR Master Mix (2×) 1 mL 1 mL × 5
ROX Reference Dye (50×) 40 μL 200 μL
ROX Reference Dye Ⅱ (50×) 40 μL 200 μL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Bulk Inquiry

Your information is safe with us. * Required Fields.

Product Name



Applicant Name *


Email Address *

Phone Number *


Organization Name *

Department *


Requested quantity *

Country or Region *



Bulk Inquiry

Inquiry Information

Product Name:
SYBR Green qPCR Master Mix
Cat. No.:
MCE Japan Authorized Agent: