1. MCE Kits
  2. Molecular Biology
  3. PCR & qPCR
  4. Real-time PCR
  5. SYBR Green qPCR Master Mix

SYBR Green qPCR Master Mix 

Cat. No.: HY-K0501
Manual SDS

MCE qPCR Master Mix is provided as a simple-to-use, stabilized 2× formulation that includes all components for qPCR except sample DNA, primers and water.

SYBR Green qPCR Master Mix
Size Price Stock Quantity
Free Sample (500 μL)   Apply Now  
1 mL USD 55 In-stock
Estimated Time of Arrival: December 31
5 mL USD 185 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

Customer Validation

mRNA expression of AB, SO, CD, OC, NA and GP genes in H-GFP and H-5A cell lines assessed by RT-qPCR.

Relative expression of MDM2, p21, Survivin, PUMA, BAX and Bcl2 genes in N-3a treated cells compared to controls.

  • Description

  • Storage

  • Components

  • Documentation

& Advantages

The Super Hot-Start SYBR Green qPCR Master Mix is a ready-to-use qPCR cocktail. It contains a novel Taq DNA Polymerase, unique hot start reagents, optimized buffer, SYBR Green I, dNTPs, PCR Enhancer and PCR stabilizer. qPCR Master Mix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

MCE qPCR Master Mix can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability. Two separate tubes of ROX Reference Dye are included for use with instruments that require a high or low level of reference dye for rectify the error of fluorescence signals between different wells.


1. Ready to use.

2. Optimized buffer enhances specificity and reduces primer-dimer formation.

3. High sensitivity, specificity and reliability.

4. Passive reference dyes are provided for different qPCR instruments.


Stored at -20°C protecting from light, and is stable for up to 18 months. Avoid repetitive freeze-thaw cycles while using. For immediate use, components may be stored at 2-8°C.

Components HY-K0501-100rxns HY-K0501-500rxns
SYBR Green qPCR Master Mix (2×) 1 mL 1 mL × 5
ROX Reference Dye (50×) 40 μL 200 μL
ROX Reference Dye Ⅱ (50×) 40 μL 200 μL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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