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Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

492 Cited Publications
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Based on 492 publication(s) in Google Scholar

Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs).The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

  • Storage :
    -20℃, 2 years.
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) Product Image
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Description & Advantages

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.

MCE Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs). It is used to maintain the phosphorylation status of proteins. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.

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Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH<sub>2</sub>O) Price Comparison

Protocol

Thaw at room temperature, add the inhibitor mixture to the lysate at 1:100 (v/v), and mix thoroughly.

Storage

-20℃, 2 years.

Components

Cat. No. Product Name Package
HY-K0022-1 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL
HY-K0022-10 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 10
HY-K0022-50 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 50
HY-K0022-100 mL Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) 1 mL × 100

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100 mg

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  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Feb 10;10(1):59. [Abstract]

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O). Western blot images of representative death genes expression in the vehicle and IFN-γ treated mouse primary cardiomyocytes.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 Nov;21(11):1251-1265. [Abstract]

    The cells were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
    Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Mar 14;56(3):620-634.e11. [Abstract]

    After chronic SDS induction with or without idazoxan administration (1 mg/kg i.p.) for 7 days, mice were used for SIT testing (Ci), measuring DBH activity (Cii) and NE concentration (Ciii) in the LC, analyzing protein expression of LBP and GAPDH in the brain (Civ and Cv), determining protein expression of BDNF, SYN, and GAPDH in the hippocampus (Cvi), and performing Golgi-Cox staining of dendritic ridge density in the CA3 (Cvii). n = 6–8, and data are representative of at least two independent experiments.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2023 May;26(5):751-764. [Abstract]

    Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322. [Abstract]

    Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.

Assay Types
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
    1/16

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2025 Feb 10;10(1):59. [Abstract]

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O). Western blot images of representative death genes expression in the vehicle and IFN-γ treated mouse primary cardiomyocytes.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
    1/16

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 Nov;21(11):1251-1265. [Abstract]

    The cells were lysed with RIPA buffer in the presence of Protease Inhibitor Cocktail (HY-K0010) and Phosphatase inhibitor cocktail II (HY-K0022) and boiled for 10 min.
    Immunoblot analysis of OVA or TRP2 levels in the mitochondria of B16-F10 cells or constructed stable B16-F10 cell lines with mitochondria bearing model antigen (OVA or TRP2). Anti-VDAC1 was used as a loading control.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
    1/16

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Immunity. 2023 Mar 14;56(3):620-634.e11. [Abstract]

    After chronic SDS induction with or without idazoxan administration (1 mg/kg i.p.) for 7 days, mice were used for SIT testing (Ci), measuring DBH activity (Cii) and NE concentration (Ciii) in the LC, analyzing protein expression of LBP and GAPDH in the brain (Civ and Cv), determining protein expression of BDNF, SYN, and GAPDH in the hippocampus (Cvi), and performing Golgi-Cox staining of dendritic ridge density in the CA3 (Cvii). n = 6–8, and data are representative of at least two independent experiments.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
    1/16

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Nat Neurosci. 2023 May;26(5):751-764. [Abstract]

    Immunoblots of BDNF expression in thalamus in MRS by Pro. Top, representative western blots. Bottom, data analysis and summary. n = 3 mice in each group.

  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
    1/16

    Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Aug 30;6(1):322. [Abstract]

    Western blot analysis showed overexpression of VAV2 and γ-H2AX in ESCC cells treated with radiation (10 Gy), which is time- and dose-dependent.

  • Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) Product Image
    1/16
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Experimental Validation Results for Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O)
  • Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH2O) Product Image