1. MCE Kits
  2. Protein Biology
  3. Protein Sample Preparation
  4. Phosphatase Inhibitor Cocktail
  5. Phosphatase Inhibitor Cocktail II

Phosphatase Inhibitor Cocktail II  (100× in ddH₂O)

Cat. No.: HY-K0022
Manual COA SDS

Phosphatase Inhibitor Cocktail II effectively inhibits acid and alkaline phosphatases as well as protein tyrosine phosphatases(PTPs).

Phosphatase Inhibitor Cocktail II
Size (Pre-dissolved in ddH₂O) Price Stock Quantity
Free Sample (100 μL)   Apply Now  
1 mL USD 38 In-stock
Estimated Time of Arrival: December 31
1 mL × 10 USD 265 In-stock
Estimated Time of Arrival: December 31
1 mL × 50 USD 1106 In-stock
Estimated Time of Arrival: December 31
1 mL × 100 USD 1660 In-stock
Estimated Time of Arrival: December 31

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  • Description

  • Price Comparison

  • Storage

  • Protocol

  • Components

  • Documentation

& Advantages

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of five phosphatase inhibitors for the inhibition of acid and alkaline phosphatases as well as protein tyrosine phosphatases (PTPs). Suitable for use with cell lysates and tissue extracts, including samples containing detergents. Provided as 1 ml vial containing the following components: Imidazole (200 mM), Sodium Fluoride (100 mM), Sodium Molybdate (115 mM), Sodium Orthovanadate (100 mM), and Sodium Tartrate Dihydrate (400 mM).

Phosphatase Inhibitor Cocktail II has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in ddH2O.

Price Comparison
Phosphatase Inhibitor Cocktail II Price Comparison
The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Ingredient M.W. 100x Conc. Target
Sodium Fluoride 42.0 100 mM Acid phosphatases
Sodium Orthovanadate 183.9 100 mM Alkaline phosphatases, PTPs, ATPases
Sodium Tartrate Dihydrate 230.1 400 mM Acid phosphatases
Sodium Molybdate 205.9 115 mM Acid and phosphoprotein phosphatases
Imidazole 68.1 200 mM Alkaline phosphatases
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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