2. Academic Validation
  3. Identification and characterization of long non-coding RNA Carip in modulating spatial learning and memory

Identification and characterization of long non-coding RNA Carip in modulating spatial learning and memory

  • Cell Rep. 2022 Feb 22;38(8):110398. doi: 10.1016/j.celrep.2022.110398.
Xiaoli Cui 1,  Ruijin Zhang 1,  Ye Yang 2,  Erzhong Wu 1,  Yiheng Tang 1,  Zhihua Zhao 2,  Chao Li 1,  Lei Yang 1,  Xueyi Teng 1,  Yanzhen Ye 2,  Ya Cui 3,  Feng Xu 1,  Zewen Su 1,  Dongpeng Wang 3,  Dongdong Zhang 3,  Yan Yang 1,  Jianyuan Sun 4,  Jianjun Luo 5,  Shuli Zhang 6,  Runsheng Chen 7,  Jianzhong Jeff Xi 8
Affiliations

Affiliations

  • 1 Key Laboratory of RNA Biology, State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • 2 Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100871, China.
  • 3 Key Laboratory of RNA Biology, State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • 4 Key Laboratory of RNA Biology, State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • 5 Key Laboratory of RNA Biology, State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: [email protected].
  • 6 Key Laboratory of RNA Biology, State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: [email protected].
  • 7 Key Laboratory of RNA Biology, State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: [email protected].
  • 8 Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing 100871, China; State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100871, China. Electronic address: [email protected].
PMID: 35196493 DOI: 10.1016/j.celrep.2022.110398
Abstract

CaMKII has long been known to be a key effector for synaptic plasticity. Recent studies have shown that a variety of modulators interact with the subunits of CaMKII to regulate the long-term potentiation (LTP) of hippocampal neurons. However, whether long non-coding RNAs modulate the activity of CaMKII and affect synaptic plasticity is still elusive. Here, we identify a previously uncharacterized long non-coding RNA Carip that functions as a scaffold, specifically interacts with CaMKIIβ, and regulates the phosphorylation of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-d-aspartate (NMDA) receptor subunits in the hippocampus. The absence of Carip causes dysfunction of synaptic transmission and attenuates LTP in hippocampal CA3-CA1 synapses, which further leads to impairment of spatial learning and memory. In summary, our findings demonstrate that Carip modulates long-term synaptic plasticity by changing AMPA receptor and NMDA receptor activities, thereby affecting spatial learning and memory in mice.

Keywords

CaMKIIβ; Carip; LTP; hippocampal neurons; learning and memory.

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