Annexin V-iFluor 488/PI Apoptosis Detection Kit
Based on 1 publication(s) in Google Scholar
MCE Annexin V-iFluor 488/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. After staining, live cells show little or no fluorescence (Annexin V-/PI-),, early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).
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Storage :
4°C, 2 years. Protect from light.
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Description & Advantages
MCE Annexin V-iFluor 488/PI Apoptosis Detection Kit provides a rapid and convenient method to detect cell apoptosis and necrosis. In normal live cells, Phosphatidylserine (PS) is located on the cytoplasmic surface of the cell membrane. Upon initiation of apoptosis, PS translocates from the inner to the outer leaflet of the membrane. Annexin V is a 35-36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with iFluor 488 can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium Iodide (PI) is a cell-membrane impermeable dye to live cells and early apoptosis cells, but stains late apoptosis cells and necrosis cells with red fluorescence. After staining cells with Annexin V-iFluor 488 and PI, live cells show little or no fluorescence (Annexin V-/PI-), early apoptosis cells show green fluorescence (Annexin V+/PI-), late apoptosis cells and necrosis cells show red and green fluorescence (Annexin V+/PI+).
Protocol
1. Cell staining
1. Collect approximately 1 × 105-5 × 105 cells, centrifuge at 300 × g for 5 min at 4°C, discard the supernatant, and wash once with pre-cooled PBS.
2. Gently resuspend cells in 100 μL 1× Annexin V Binding Buffer.
3. Add 5 μL Annexin V-iFluor 488 and 10 μL PI Staining Solution and mix gently.
4. Incubate for 15 min at room temperature protected from light.
5. Add 400 μL 1× Annexin V Binding Buffer and mix gently.
6. Analyze by flow cytometry preferably within 1 h.
Interpretation
Viable cells are Annexin V-negative/counterstain-negative; early apoptotic cells are Annexin V-positive/counterstain-negative; late apoptotic or necrotic cells are positive for both Annexin V and PI Staining Solution.
Note: Protect stained cells from light and analyze promptly. For blood-derived samples, remove platelets thoroughly because platelet phosphatidylserine may bind Annexin V and interfere with the result.
Publications
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Journal Impact Factor
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Most Recent
Storage
4°C, 2 years.
Protect from light.
Components
| Components | HY-K1080-25T | HY-K1080-50T | HY-K1080-100T |
|---|---|---|---|
| Annexin V-iFluor 488 | 125 μL | 250 μL | 500 μL |
| 10× Binding Buffer | 2 mL | 2 × 2 mL | 3 × 2 mL |
| PI Solution | 125 μL | 250 μL | 500 μL |
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